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Literature summary for 1.8.2.5 extracted from
- Thiosulfate reductase isolated from Desulfotomaculum nigrificans (1969), J. Bacteriol., 98, 429-433.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-hydroxymercuribenzoate | activity can be restored by cysteine | Desulfotomaculum nigrificans |  |
| iodoacetamide | - |
Desulfotomaculum nigrificans | |
| N-ethylmaleimide | - |
Desulfotomaculum nigrificans | |
| sulfite | - |
Desulfotomaculum nigrificans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfotomaculum nigrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Desulfotomaculum nigrificans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 33% loss of activity within 2 weeks | Desulfotomaculum nigrificans |
| -20°C, lyophilized preparation, stable for at least 1 month | Desulfotomaculum nigrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | with inner- and outer-labeled 35S-thiosulfate, the enzyme reduces only the outer sulfur atom to hydrogen sulfide. No substrates: sulfate, sulfite, tetrathionate, dithionate. No electron donors: reduced nicotinamide adenine dinucleotide, reduced nicotinamide adenine dinucleotide phosphate, reduced glutathione, or cysteine | Desulfotomaculum nigrificans | ? | - |
? | |
| thiosulfate + reduced methyl viologen | - |
Desulfotomaculum nigrificans | sulfite + hydrogen sulfide + oxidized methyl viologen | - |
? | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Desulfotomaculum nigrificans |
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Release 2023.1 (January 2023)
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