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Literature summary for 1.8.2.5 extracted from
- Purification and properties of thiosulfate reductase from Desulfovibrio gigas (1975), Arch. Microbiol., 105, 249-256.
General Stability
| General Stability | Organism |
|---|---|
| enzyme loses activtiy upon freezing and thawing | Megalodesulfovibrio gigas |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 0.02 mM, 100% inhibition. Inhibition can be partially or completely reversed by cysteine | Megalodesulfovibrio gigas |  |
| Ag+ | 0.01 mM, 90% inhibition | Megalodesulfovibrio gigas | |
| Hg2+ | 0.01 mM, 100% inhibition | Megalodesulfovibrio gigas | |
| iodoacetate | 0.5 mM, 70% inhibition | Megalodesulfovibrio gigas | |
| N-ethylmaleimide | 0.5 mM, 55% inhibition | Megalodesulfovibrio gigas | |
| o-Iodosobenzoate | 0.05 mM, 52% inhibition | Megalodesulfovibrio gigas | |
| sulfite | 3 mM, 45% inhibition | Megalodesulfovibrio gigas | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
thiosulfate | 37°C, pH 7.6 | Megalodesulfovibrio gigas | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 220000 | - |
gel filtration | Megalodesulfovibrio gigas |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiosulfate + 2 ferrocytochrome c3 | Megalodesulfovibrio gigas | - |
sulfite + hydrogen sulfide + 2 ferricytochrome c3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Megalodesulfovibrio gigas | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| unstable during purification | Megalodesulfovibrio gigas |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41 | - |
37°C, pH 7.6 | Megalodesulfovibrio gigas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme catalyzes the stoichiometric production of hydrogen sulfide and sulfite from thiosulfate. The most efficient coupling is obtained with a system containing cytochromes c3. The enzyme exhibits tetrathionate reductase activity. It does not show sulfite reductase activity | Megalodesulfovibrio gigas | ? | - |
? | |
| thiosulfate + 2 ferrocytochrome c3 | - |
Megalodesulfovibrio gigas | sulfite + hydrogen sulfide + 2 ferricytochrome c3 | - |
? | |
| thiosulfate + reduced methyl viologen | - |
Megalodesulfovibrio gigas | sulfite + hydrogen sulfide + oxidized methyl viologen | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8 | - |
Megalodesulfovibrio gigas |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
8% of maximum activity | Megalodesulfovibrio gigas |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c3 | - |
Megalodesulfovibrio gigas |
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