BRENDA - Enzyme Database
show all sequences of 1.8.2.3

Sulfide-cytochrome c reductase (flavocytochrome c)

Yamanaka, T.; Methods Enzymol. 243, 463-472 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
-
Allochromatium vinosum
cyanide
-
Chlorobaculum thiosulfatiphilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Chlorobaculum thiosulfatiphilum
21000
-
1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Allochromatium vinosum
46000
-
1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Allochromatium vinosum
47000
-
1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Chlorobaculum thiosulfatiphilum
58000
-
gel filtration
Chlorobaculum thiosulfatiphilum
72000
-
gel filtration
Allochromatium vinosum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Allochromatium vinosum
-
-
-
Chlorobaculum thiosulfatiphilum
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography
Allochromatium vinosum
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography
Chlorobaculum thiosulfatiphilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfide + oxidized benzyl viologen
-
716105
Allochromatium vinosum
sulfur + reduced benzyl viologen + H+
-
-
-
?
sulfide + oxidized benzyl viologen
-
716105
Chlorobaculum thiosulfatiphilum
sulfur + reduced benzyl viologen + H+
-
-
-
?
sulfide + oxidized flavocytochrome c
-
716105
Allochromatium vinosum
sulfur + reduced flavocytochrome c + H+
-
-
-
?
sulfide + oxidized flavocytochrome c
-
716105
Chlorobaculum thiosulfatiphilum
sulfur + reduced flavocytochrome c + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Allochromatium vinosum
heterodimer
1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Chlorobaculum thiosulfatiphilum
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
covalently bound, the enzyme contains one molecule of FAD per molecule
Allochromatium vinosum
FAD
covalently bound, the enzyme contains one molecule of FAD per molecule
Chlorobaculum thiosulfatiphilum
heme
covalently bound heme c, the enzyme contains two molecules of heme c per molecule
Allochromatium vinosum
heme
covalently bound heme c, the enzyme contains one molecule of heme c per molecule
Chlorobaculum thiosulfatiphilum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Allochromatium vinosum
isoelectric focusing
-
5.1
Chlorobaculum thiosulfatiphilum
isoelectric focusing
-
6.7
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
covalently bound, the enzyme contains one molecule of FAD per molecule
Allochromatium vinosum
FAD
covalently bound, the enzyme contains one molecule of FAD per molecule
Chlorobaculum thiosulfatiphilum
heme
covalently bound heme c, the enzyme contains two molecules of heme c per molecule
Allochromatium vinosum
heme
covalently bound heme c, the enzyme contains one molecule of heme c per molecule
Chlorobaculum thiosulfatiphilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
-
Allochromatium vinosum
cyanide
-
Chlorobaculum thiosulfatiphilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11000
-
1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Chlorobaculum thiosulfatiphilum
21000
-
1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Allochromatium vinosum
46000
-
1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Allochromatium vinosum
47000
-
1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Chlorobaculum thiosulfatiphilum
58000
-
gel filtration
Chlorobaculum thiosulfatiphilum
72000
-
gel filtration
Allochromatium vinosum
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography
Allochromatium vinosum
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography
Chlorobaculum thiosulfatiphilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfide + oxidized benzyl viologen
-
716105
Allochromatium vinosum
sulfur + reduced benzyl viologen + H+
-
-
-
?
sulfide + oxidized benzyl viologen
-
716105
Chlorobaculum thiosulfatiphilum
sulfur + reduced benzyl viologen + H+
-
-
-
?
sulfide + oxidized flavocytochrome c
-
716105
Allochromatium vinosum
sulfur + reduced flavocytochrome c + H+
-
-
-
?
sulfide + oxidized flavocytochrome c
-
716105
Chlorobaculum thiosulfatiphilum
sulfur + reduced flavocytochrome c + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
1 * 46000 + 1 * 21000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Allochromatium vinosum
heterodimer
1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
Chlorobaculum thiosulfatiphilum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Allochromatium vinosum
isoelectric focusing
-
5.1
Chlorobaculum thiosulfatiphilum
isoelectric focusing
-
6.7
Other publictions for EC 1.8.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714154
Quentmeier
Sulfide dehydrogenase activity ...
Paracoccus pantotrophus
Biochemistry
43
14696-14703
2004
-
-
-
-
-
-
4
2
-
-
3
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
1
1
-
-
1
2
-
-
-
-
-
1
-
-
-
-
4
2
2
-
-
3
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
715342
Kostanjevecki
A membrane-bound flavocytochro ...
Ectothiorhodospira shaposhnikovii
J. Bacteriol.
182
3097-3103
2000
-
-
-
-
-
-
-
-
1
-
2
-
-
6
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
713981
Visser
A novel membrane-bound flavocy ...
Thiobacillus sp., Thiobacillus sp. W5
Arch. Microbiol.
167
295-301
1997
-
-
-
-
-
-
1
2
1
-
2
-
-
7
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
1
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
716866
Van Driessche
Covalent structure of the flav ...
Allochromatium vinosum
Protein Sci.
5
1753-1764
1996
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716105
Yamanaka
-
Sulfide-cytochrome c reductase ...
Allochromatium vinosum, Chlorobaculum thiosulfatiphilum
Methods Enzymol.
243
463-472
1994
-
-
-
-
-
-
2
-
-
-
6
-
-
2
-
-
2
-
-
-
-
-
4
2
-
-
-
-
-
-
-
4
-
2
-
-
-
-
4
-
-
-
-
2
-
-
-
-
6
-
-
-
-
2
-
-
-
-
4
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
716916
Chen
The structure of flavocytochro ...
Allochromatium vinosum
Science
266
430-432
1994
-
-
-
1
-
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
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-
2
-
-
-
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-
-
2
1
-
-
-
-
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-
-
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3
-
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-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714363
Gray
-
The role of a cytochrome c-552 ...
Allochromatium vinosum
Biochim. Biophys. Acta
680
290-296
1982
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
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-
1
-
-
2
1
-
-
-
-
-
-
-
-
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-
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-
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-
4
-
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-
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-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715409
Fukumori
Flavocytochrome c of Chromatiu ...
Allochromatium vinosum
J. Biochem.
85
1405-1414
1979
-
-
-
-
-
-
2
1
-
-
3
-
-
5
-
-
-
-
-
-
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
3
-
-
-
-
-
-
-
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
714361
Kusai
The oxidation mechanisms of th ...
Chlorobium vibrioforme f. thiosulfatophilum
Biochim. Biophys. Acta
325
304-314
1973
1
-
-
-
-
-
4
-
-
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-
1
-
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1
-
-
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-
1
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-
1
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2
-
-
1
1
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-
2
-
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-
1
4
-
-
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1
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1
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1
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