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Literature summary for 1.8.2.3 extracted from
- The oxidation mechanisms of thiosulphate and sulphide in Chlorobium thiosulphatophilum: roles of cytochrome c-551 and cytochrome c-553 (1973), Biochim. Biophys. Acta, 325, 304-314.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | neither FAD nor FMN activate sulfide-cytochrome c reduction catalysed by cytochrome c-553 | Prosthecochloris vibrioformis f. thiosulfatophilum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Atebrin | 60% inhibition at 0.1 mM | Prosthecochloris vibrioformis f. thiosulfatophilum |  |
| cyanide | 80% inhibition at 0.001 mM | Prosthecochloris vibrioformis f. thiosulfatophilum | |
| additional information | not inhibited by EDTA, o-phenanthroline, and CO | Prosthecochloris vibrioformis f. thiosulfatophilum | |
| NaN3 | 7% inhibition at 10 mM | Prosthecochloris vibrioformis f. thiosulfatophilum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prosthecochloris vibrioformis f. thiosulfatophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and DEAE-cellulose column chromatography | Prosthecochloris vibrioformis f. thiosulfatophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydrogen sulfide + ferricytochrome c 555 | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | sulfur + ferrocytochrome c 555 + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sulphide-cytochrome c reductase | - |
Prosthecochloris vibrioformis f. thiosulfatophilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
the enzyme is completely inactive after 2 min at 80°C | Prosthecochloris vibrioformis f. thiosulfatophilum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | covalently-bound | Prosthecochloris vibrioformis f. thiosulfatophilum | |
| heme | - |
Prosthecochloris vibrioformis f. thiosulfatophilum | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
in 0.1 M Tris-HCl buffer, pH 7.4, at 20°C | Prosthecochloris vibrioformis f. thiosulfatophilum | cyanide | |
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