BRENDA - Enzyme Database
show all sequences of 1.8.2.3

Sulfide dehydrogenase activity of the monomeric flavoprotein SoxF of Paracoccus pantotrophus

Quentmeier, A.; Hellwig, P.; Bardischewsky, F.; Wichmann, R.; Friedrich, C.; Biochemistry 43, 14696-14703 (2004)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
1 mM cyanide inhibits SoxF activity at pH 6.0 by 25% and at pH 8.0 by 92%
Paracoccus pantotrophus
reduced heart cytochrome c
competitive product inhibition
Paracoccus pantotrophus
sulfite
preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity)
Paracoccus pantotrophus
sulfur
-
Paracoccus pantotrophus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
Sulfide
at pH 6.0 and 30C
Paracoccus pantotrophus
0.116
-
oxidized heart cytochrome c
at pH 6.0 and 30C
Paracoccus pantotrophus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42010
-
calculated from amino acid sequence
Paracoccus pantotrophus
42797
-
1 * 42797, electrospray mass spectrometry
Paracoccus pantotrophus
42832
-
1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
Paracoccus pantotrophus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paracoccus pantotrophus
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography
Paracoccus pantotrophus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
SoxF does not catalyze thiosulfate- or sulfite-dependent cytochrome c reduction
714154
Paracoccus pantotrophus
?
-
-
-
-
sulfide + oxidized heart cytochrome c
-
714154
Paracoccus pantotrophus
sulfur + reduced heart cytochrome c + H+
sulfur or polysulfide as product
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 42797, electrospray mass spectrometry; 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
Paracoccus pantotrophus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.9
-
oxidized heart cytochrome c
at pH 6.0 and 30C
Paracoccus pantotrophus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Paracoccus pantotrophus
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Paracoccus pantotrophus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0013
-
sulfur
at pH 6.0 and 30C
Paracoccus pantotrophus
0.0072
-
reduced heart cytochrome c
at pH 6.0 and 30C
Paracoccus pantotrophus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Paracoccus pantotrophus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
1 mM cyanide inhibits SoxF activity at pH 6.0 by 25% and at pH 8.0 by 92%
Paracoccus pantotrophus
reduced heart cytochrome c
competitive product inhibition
Paracoccus pantotrophus
sulfite
preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity)
Paracoccus pantotrophus
sulfur
-
Paracoccus pantotrophus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0013
-
sulfur
at pH 6.0 and 30C
Paracoccus pantotrophus
0.0072
-
reduced heart cytochrome c
at pH 6.0 and 30C
Paracoccus pantotrophus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
Sulfide
at pH 6.0 and 30C
Paracoccus pantotrophus
0.116
-
oxidized heart cytochrome c
at pH 6.0 and 30C
Paracoccus pantotrophus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42010
-
calculated from amino acid sequence
Paracoccus pantotrophus
42797
-
1 * 42797, electrospray mass spectrometry
Paracoccus pantotrophus
42832
-
1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
Paracoccus pantotrophus
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation and Q-Sepharose column chromatography
Paracoccus pantotrophus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
SoxF does not catalyze thiosulfate- or sulfite-dependent cytochrome c reduction
714154
Paracoccus pantotrophus
?
-
-
-
-
sulfide + oxidized heart cytochrome c
-
714154
Paracoccus pantotrophus
sulfur + reduced heart cytochrome c + H+
sulfur or polysulfide as product
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 42797, electrospray mass spectrometry; 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
Paracoccus pantotrophus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.9
-
oxidized heart cytochrome c
at pH 6.0 and 30C
Paracoccus pantotrophus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Paracoccus pantotrophus
Other publictions for EC 1.8.2.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
714154
Quentmeier
Sulfide dehydrogenase activity ...
Paracoccus pantotrophus
Biochemistry
43
14696-14703
2004
-
-
-
-
-
-
4
2
-
-
3
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
1
1
-
-
1
2
-
-
-
-
-
1
-
-
-
-
4
2
2
-
-
3
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
715342
Kostanjevecki
A membrane-bound flavocytochro ...
Ectothiorhodospira shaposhnikovii
J. Bacteriol.
182
3097-3103
2000
-
-
-
-
-
-
-
-
1
-
2
-
-
6
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
713981
Visser
A novel membrane-bound flavocy ...
Thiobacillus sp., Thiobacillus sp. W5
Arch. Microbiol.
167
295-301
1997
-
-
-
-
-
-
1
2
1
-
2
-
-
7
-
-
1
-
-
-
-
-
2
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
1
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
716866
Van Driessche
Covalent structure of the flav ...
Allochromatium vinosum
Protein Sci.
5
1753-1764
1996
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716105
Yamanaka
-
Sulfide-cytochrome c reductase ...
Allochromatium vinosum, Chlorobaculum thiosulfatiphilum
Methods Enzymol.
243
463-472
1994
-
-
-
-
-
-
2
-
-
-
6
-
-
2
-
-
2
-
-
-
-
-
4
2
-
-
-
-
-
-
-
4
-
2
-
-
-
-
4
-
-
-
-
2
-
-
-
-
6
-
-
-
-
2
-
-
-
-
4
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
716916
Chen
The structure of flavocytochro ...
Allochromatium vinosum
Science
266
430-432
1994
-
-
-
1
-
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714363
Gray
-
The role of a cytochrome c-552 ...
Allochromatium vinosum
Biochim. Biophys. Acta
680
290-296
1982
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
2
1
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-
-
-
-
-
-
-
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-
-
-
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-
4
-
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-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715409
Fukumori
Flavocytochrome c of Chromatiu ...
Allochromatium vinosum
J. Biochem.
85
1405-1414
1979
-
-
-
-
-
-
2
1
-
-
3
-
-
5
-
-
-
-
-
-
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
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-
3
-
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-
-
-
-
-
5
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
714361
Kusai
The oxidation mechanisms of th ...
Chlorobium vibrioforme f. thiosulfatophilum
Biochim. Biophys. Acta
325
304-314
1973
1
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
1
1
-
-
2
-
-
-
1
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
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-
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-
-