Literature summary for 1.8.2.2 extracted from

Liu, Y.; Denkmann, K.; Kosciow, K.; Dahl, C.; Kelly, D.
Tetrathionate stimulated growth of Campylobacter jejuni identifies a new type of bi-functional tetrathionate reductase (TsdA) that is widely distributed in bacteria (2013), Mol. Microbiol., 88, 173-188.

Search for more literature for 1.8.2.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	ferricyanide	S0.5 value, pH 4.0, 42°C	Campylobacter jejuni
0.2	-	thiosulfate	pH 4.0, 42°C	Campylobacter jejuni

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	-	bifunctional tetrathionate reductase/thiosulfate dehydrogenase	-
Campylobacter jejuni NCTC 11828	-	bifunctional tetrathionate reductase/thiosulfate dehydrogenase	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 thiosulfate + 2 ferricyanide	stoichiometric ratio of conversion is of 1 mole tetrathionate to 2 mol initial thiosulfate. Enzyme can couple thiosulfate oxidation to electron transport to oxygen	Campylobacter jejuni	tetrathionate + 2 ferrocyanide	-	?
2 thiosulfate + 2 ferricyanide	stoichiometric ratio of conversion is of 1 mole tetrathionate to 2 mol initial thiosulfate. Enzyme can couple thiosulfate oxidation to electron transport to oxygen	Campylobacter jejuni NCTC 11828	tetrathionate + 2 ferrocyanide	-	?

Synonyms

Synonyms	Comment	Organism
C8J_0815	locus name	Campylobacter jejuni
TsdA	-	Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
42	-	-	Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
604	-	thiosulfate	pH 4.0, 42°C	Campylobacter jejuni

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	substrate ferricyanide	Campylobacter jejuni

Cofactor

Cofactor	Comment	Organism	Structure
heme	sequence shows two Cys-X2-Cys-His heme c-binding motifs	Campylobacter jejuni

General Information

General Information	Comment	Organism
physiological function	growth of the microaerophilic mucosal pathogen Campylobacter jejuni under oxygen-limited conditions is stimulated by tetrathionate. A tsdA null mutant still slowly reduces, but can not grow on, tetrathionate under oxygen limitation, lacks thiosulfate-dependent respiration and fails to convert thiosulfate to tetrathionate microaerobically. Physiological role for Campylobacter jejuni TsdA is a tetrathionate reductase	Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
302	-	thiosulfate	pH 4.0, 42°C	Campylobacter jejuni

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)