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Literature summary for 1.8.2.1 extracted from

  • Qiu, J.A.; Wilson, H.L.; Pushie, M.J.; Kisker, C.; George, G.N.; Rajagopalan, K.V.
    The structures of the C185S and C185A mutants of sulfite oxidase reveal rearrangement of the active site (2010), Biochemistry, 49, 3989-4000.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TP1000 cells Gallus gallus

Crystallization (Commentary)

Crystallization (Comment) Organism
the C185S variant is crystallized by the hanging drop vapor diffusion method using 15% (v/v) 2-methyl-2,4-pentanediol, 2% PEG 4000 (w/v), and 100 mM sodium acetate (pH 5.0) and in the presence of sulfite using 2% PEG 4000 (w/v), 10% MPD (v/v), 100 mM sodium acetate (pH 5.0) and 0.5 mM sodium sulfite. The C185A variant is crystallized by the sitting drop vapor diffusion method, using 15% (w/v) PEG 4000, 150 mM (NH4)2SO4, and 100 mM MES (pH 6.0) Gallus gallus

Protein Variants

Protein Variants Comment Organism
C185A the active site of the mutant is essentially catalytically inactive with ferricyctochrome c or ferricyanide as electron acceptor Gallus gallus
C185S the active site of the mutant is essentially catalytically inactive with ferricyctochrome c or ferricyanide as electron acceptor Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + ferricytochrome c + H2O Gallus gallus ferricytochrome c is the physiological electron acceptor sulfate + ferrocytochrome c + H+
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus P07850
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography and Superdex-200 gel filtration Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricyanide + H2O
-
Gallus gallus sulfate + ferrocyanide + H+
-
?
sulfite + ferricytochrome c + H2O ferricytochrome c is the physiological electron acceptor Gallus gallus sulfate + ferrocytochrome c + H+
-
?

Synonyms

Synonyms Comment Organism
sulfate oxidase
-
Gallus gallus

Cofactor

Cofactor Comment Organism Structure
heme
-
Gallus gallus
molybdopterin
-
Gallus gallus