BRENDA - Enzyme Database show
show all sequences of 1.8.2.1

Kinetic and structural evidence for the importance of Tyr236 for the integrity of the Mo active site in a bacterial sulfite dehydrogenase

Kappler, U.; Bailey, S.; Feng, C.; Honeychurch, M.J.; Hanson, G.R.; Bernhardt, P.V.; Tollin, G.; Enemark, J.H.; Biochemistry 45, 9696-9705 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain DH5alpha
Starkeya novella
Crystallization (Commentary)
Crystallization
Organism
-
Starkeya novella
Engineering
Amino acid exchange
Commentary
Organism
Y236F
reduced turnover rates and substrate affinity as well as an altered reactivity toward molecular oxygen as an electron acceptor, unlike the wild type enzyme the mutant enzyme is reoxidized quickly in the presence of molecular oxygen
Starkeya novella
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Molybdenum
-
Starkeya novella
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
wild type enzyme, analytical ultracentrifugation
Starkeya novella
43000
-
mutant enzyme Y236F, analytical ultracentrifugation
Starkeya novella
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Starkeya novella
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE anion exchange column chromatography
Starkeya novella
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfite + ferricytochrome c + H2O
-
667744
Starkeya novella
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
-
Starkeya novella
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.4
-
sulfite
mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0
Starkeya novella
0.8
-
sulfite
mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0
Starkeya novella
33.51
-
sulfite
wild type enzyme, in 20 mM bis-Tris-acetate buffer pH 6.0
Starkeya novella
36.19
-
sulfite
mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0
Starkeya novella
52.51
-
sulfite
mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0
Starkeya novella
333.7
-
sulfite
wild type enzyme, in 20 mM Tris-acetate buffer pH 8.0
Starkeya novella
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.4
wild type enzyme
Starkeya novella
7.2
8.64
mutant enzyme Y236F
Starkeya novella
Cofactor
Cofactor
Commentary
Organism
Structure
heme c
-
Starkeya novella
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain DH5alpha
Starkeya novella
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme c
-
Starkeya novella
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Starkeya novella
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Y236F
reduced turnover rates and substrate affinity as well as an altered reactivity toward molecular oxygen as an electron acceptor, unlike the wild type enzyme the mutant enzyme is reoxidized quickly in the presence of molecular oxygen
Starkeya novella
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Molybdenum
-
Starkeya novella
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
wild type enzyme, analytical ultracentrifugation
Starkeya novella
43000
-
mutant enzyme Y236F, analytical ultracentrifugation
Starkeya novella
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE anion exchange column chromatography
Starkeya novella
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sulfite + ferricytochrome c + H2O
-
667744
Starkeya novella
sulfate + ferrocytochrome c + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
-
Starkeya novella
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.4
-
sulfite
mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0
Starkeya novella
0.8
-
sulfite
mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0
Starkeya novella
33.51
-
sulfite
wild type enzyme, in 20 mM bis-Tris-acetate buffer pH 6.0
Starkeya novella
36.19
-
sulfite
mutant enzyme Y236F, in 20 mM bis-Tris-acetate buffer pH 6.0
Starkeya novella
52.51
-
sulfite
mutant enzyme Y236F, in 20 mM Tris-acetate buffer pH 8.0
Starkeya novella
333.7
-
sulfite
wild type enzyme, in 20 mM Tris-acetate buffer pH 8.0
Starkeya novella
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
9.4
wild type enzyme
Starkeya novella
7.2
8.64
mutant enzyme Y236F
Starkeya novella
Other publictions for EC 1.8.2.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743146
Kalimuthu
Catalytic voltammetry of the ...
Sinorhizobium meliloti
J. Phys. Chem. B
118
7091-7099
2014
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1
2
1
1
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7
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1
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3
1
1
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1
1
2
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1
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1
1
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3
-
3
1
1
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1
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2
1
1
-
-
-
-
-
-
-
724418
Rapson
Short circuiting a sulfite oxi ...
Starkeya novella
Biochim. Biophys. Acta
1807
108-118
2011
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1
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4
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710857
Kalimuthu
Highly sensitive and stable el ...
Starkeya novella
Anal. Chem.
82
7374-7379
2010
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1
1
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1
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1
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711223
Johnson-Winters
Effects of interdomain tether ...
Homo sapiens
Biochemistry
49
1290-1296
2010
-
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3
-
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7
1
1
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1
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1
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1
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1
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7
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1
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1
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3
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7
1
1
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1
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1
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1
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7
-
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6
6
711242
Qiu
The structures of the C185S an ...
Gallus gallus
Biochemistry
49
3989-4000
2010
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-
1
1
2
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1
-
1
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1
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1
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2
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2
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1
2
1
2
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1
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1
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2
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711780
Spricigo
-
Sulfite biosensor based on osm ...
Homo sapiens
Colloids Surf. A Physicochem. Eng. Asp.
354
314-319
2010
-
1
1
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1
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1
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1
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1
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1
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-
724587
Ogawa
Biochemical studies of a soxF- ...
Chlorobaculum tepidum
Biosci. Biotechnol. Biochem.
74
771-780
2010
-
-
-
-
-
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-
1
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3
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1
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1
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1
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1
1
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1
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1
1
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-
700633
Astashkin
Exchangeable oxygens in the vi ...
Starkeya novella
Phys. Chem. Chem. Phys.
11
6733-6742
2009
-
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1
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712157
Hernandez-Marin
Theoretical study of the oxida ...
Gallus gallus
Inorg. Chem.
48
1323-1333
2009
-
-
-
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1
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1
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722663
Bailey
Molecular basis for enzymatic ...
Starkeya novella
J. Biol. Chem.
284
2053-2063
2009
-
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1
3
-
-
32
-
1
-
1
-
1
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2
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30
1
1
-
2
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2
1
3
-
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32
-
1
-
1
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2
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30
1
1
-
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32
32
691057
Rapson
Direct catalytic electrochemis ...
Starkeya novella
Biochim. Biophys. Acta
1777
1319-1325
2008
1
-
-
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-
4
6
-
1
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2
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1
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1
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1
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1
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1
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694023
Workun
Evolutionary persistence of th ...
Starkeya novella
Microbiol. Mol. Biol. Rev.
72
228-48
2008
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1
1
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7
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1
1
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1
1
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694036
Denger
Bacterial sulfite dehydrogenas ...
Cupriavidus necator H16, Delftia acidovorans SPH-1
Microbiology
154
256-263
2008
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2
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5
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8
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1
2
2
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1
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667714
Wilson
The G473D mutation impairs dim ...
Homo sapiens
Biochemistry
45
2149-2160
2006
-
-
1
-
4
-
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8
-
1
5
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1
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1
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2
1
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667744
Kappler
Kinetic and structural evidenc ...
Starkeya novella
Biochemistry
45
9696-9705
2006
-
-
1
1
1
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1
2
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3
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1
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1
1
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6
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2
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1
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1
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6
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668939
Doonan
Structure of the active site o ...
Starkeya novella
Inorg. Chem.
45
7488-7492
2006
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695918
Gorzynska
Inducible transcription of gen ...
Ruegeria pomeroyi
Arch. Microbiol.
185
402-406
2006
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667688
Sibille
Solution structure of the sulf ...
Escherichia coli
Biochemistry
44
9086-9095
2005
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668937
Raitsimring
Pulsed EPR studies of a bacter ...
Starkeya novella
Inorg. Chem.
44
7283-7285
2005
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5
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669353
Kappler
Molecular basis of intramolecu ...
Starkeya novella
J. Biol. Chem.
280
24999-25007
2005
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7
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657461
Kappler
Crystallization and preliminar ...
Starkeya novella
Acta Crystallogr. Sect. D
60
2070-2072
2004
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Rhodotorula sp.
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The sulfite oxidase of Thiobac ...
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Enzymes involved in the metabo ...
Thiobacillus thioparus
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Charles
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Purification and properties of ...
Starkeya novella
Biochim. Biophys. Acta
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Mechanism of thiosulfate oxida ...
Starkeya novella, Thiobacillus denitrificans
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