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Literature summary for 1.8.2.1 extracted from
- A system for the heterologous expression of complex redox proteins in Rhodobacter capsulatus: characterisation of recombinant sulphite:cytochrome c oxidoreductase from Starkeya novella (2002), FEBS Lett., 529, 208-214.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologous expression in Rhodobacter capsulatus with the expression vector pDorEX, potentially useful for heterologous expression of multi-subunit proteins containing complex redox cofactors | Starkeya novella |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
ferricytochrome c | recombinant enzyme | Starkeya novella |  |
| 0.032 | - |
sulfite | recombinant enzyme | Starkeya novella | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | the recombinant enzyme is secreted into the periplasm of Rhodobacter capsulatus | Starkeya novella | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | 0.63 per holoenzyme | Starkeya novella | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
recombinant enzyme, analytical ultracentrifugation | Starkeya novella |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Starkeya novella | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Starkeya novella |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Starkeya novella |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + ferricyanide + H2O | - |
Starkeya novella | sulfate + ferrocyanide | - |
? | |
| sulfite + ferricytochrome c + H2O | - |
Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alphabeta | Starkeya novella |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SorAB | - |
Starkeya novella |
| sulphite:cytochrome c oxidoreductase | - |
Starkeya novella |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10880 | - |
sulfite | recombinant enzyme | Starkeya novella | |
| 10880 | - |
ferricytochrome c | recombinant enzyme | Starkeya novella | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | 0.91 heme c per holoenzyme | Starkeya novella | |
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