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Literature summary for 1.8.2.1 extracted from

  • Kappler, U.; Bennett, B.; Rethmeier, J.; Schwarz, G.; Deutzmann, R.; McEwan, A.G.; Dahl, C.
    Sulfite:cytochrome c oxidoreductase from Thiobacillus novellus. Purification, characterization, and molecular biology of a heterodimeric member of the sulfite oxidase family (2000), J. Biol. Chem., 275, 13202-13212.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Starkeya novella

Inhibitors

Inhibitors Comment Organism Structure
SO42- product inhibition, mixed-type non-competitive with respect to sulfite Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
ferricytochrome c550
-
Starkeya novella
0.004
-
ferricytochrome c
-
Starkeya novella
0.027
-
sulfite
-
Starkeya novella

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Starkeya novella
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum contains 1.2 Mo atoms per holoenzyme Starkeya novella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
gel filtration Starkeya novella

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Starkeya novella

Reaction

Reaction Comment Organism Reaction ID
sulfite + 2 ferricytochrome c + H2O = sulfate + 2 ferrocytochrome c + 2 H+ ping-pong mechanism Starkeya novella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
327.9
-
-
Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricytochrome c + H2O
-
Starkeya novella sulfate + ferrocytochrome c + H+
-
?
sulfite + ferricytochrome c550 + H2O
-
Starkeya novella sulfate + ferrocytochrome c550 + H+
-
r

Cofactor

Cofactor Comment Organism Structure
cytochrome c550 enzyme reacts exclusively with cytochrome c550 Starkeya novella
heme the enzyme contains 0.8 heme groups per holoenzyme Starkeya novella

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Starkeya novella