Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | maximal actiovity at final substrate concentrations of 0.02-0.05 M at pH 7.8 | Rhodotorula sp. |
General Stability | Organism |
---|---|
treatment with 1-2 mg trypsin per 1.5 mg of protein or 0.1-0.2 mg of pronase or 1.5 mg of protein at 25°C causes decline of activity | Rhodotorula sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 5 mM, 30% inhibition | Rhodotorula sp. | |
2,2'-bipyridyl | 5 mM, 50% inhibition | Rhodotorula sp. | |
AMP | 0.01 mM, 50% inhibition | Rhodotorula sp. | |
EDTA | 10 mM Na2EDTA, 30% inhibition | Rhodotorula sp. | |
HgCl2 | 0.25 mM, complete inhibition | Rhodotorula sp. | |
NEM | 1 mM, complete inhibition | Rhodotorula sp. | |
p-hydroxymercuribenzoate | 1 mM, complete inhibition | Rhodotorula sp. | |
sodium deoxycholate | 1.0%, 80% inhibition | Rhodotorula sp. | |
Triton X-100 | 1.0%, 15% inhibition | Rhodotorula sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
sulfite | reaction with cytochrome c | Rhodotorula sp. | |
0.42 | - |
ferricyanide | - |
Rhodotorula sp. | |
1 | - |
sulfite | reaction with ferricyanide | Rhodotorula sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula sp. | - |
enzyme complex that posseses sulfite oxidase activity and thiosulfate-oxidizing activity | - |
Storage Stability | Organism |
---|---|
-20°C, in presence of 20% glycerol stable for several days, without glycerol most of the activity is lost after 48 h | Rhodotorula sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricyanide + H2O | - |
Rhodotorula sp. | sulfate + ferrocyanide + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | - |
Rhodotorula sp. | sulfate + ferrocytochrome c + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodotorula sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, 60% loss of activity | Rhodotorula sp. |
60 | - |
7 min, 70% loss of activity | Rhodotorula sp. |
100 | - |
boiling of the purified enzyme complex destroys activity completely | Rhodotorula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
reaction with native cytochrome c | Rhodotorula sp. |