General Stability | Organism |
---|---|
stable to repeated freezing and thawing | Starkeya novella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 0.01 mM, 25% inhibition, activity with ferricyanide | Starkeya novella | |
EDTA | 0.005 mM, 20% inhibition | Starkeya novella | |
HgCl2 | 0.667 mM, 56% inhibition | Starkeya novella | |
additional information | CaCl2, MgSO4, ZnSO4, CuSO4, FeCl3, NiCl2 and Na2MoO4 inhibits the reaction by 70% at 0.267 mM, this effect is apparently due to the non-enzymatic oxidation of sulfite with air | Starkeya novella | |
NaAsO2 | 31% inhibition | Starkeya novella | |
NaCl | competitive with respect to sulfite | Starkeya novella | |
NaF | 0.01 mM, 17.5% inhibition | Starkeya novella | |
NEM | 0.667 mM, 30% loss of activity | Starkeya novella | |
p-hydroxymercuribenzoate | 0.667 mM, 86% loss of activity, inhibition is completely reversed by GSH | Starkeya novella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
sulfite | pH 6.5, reaction with cytochrome c | Starkeya novella | |
0.02 | - |
ferricyanide | pH 8.0 | Starkeya novella | |
0.04 | - |
sulfite | pH 8.0, reaction with cytochrome c | Starkeya novella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricytochrome c + H2O | Starkeya novella | oxidative phosphorylation is coupled to sulfite oxidation with a low P/O ratio | sulfate + ferrocytochrome c + H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 50%, significant loss of activity | Starkeya novella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starkeya novella | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Starkeya novella |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
139.5 | - |
reaction with ferricyanide | Starkeya novella |
Storage Stability | Organism |
---|---|
-20°C, retains high activity for more than 6 months | Starkeya novella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + ferricyanide + H2O | - |
Starkeya novella | sulfate + ferrocyanide + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | specific for sulfite | Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? | |
sulfite + ferricytochrome c + H2O | oxidative phosphorylation is coupled to sulfite oxidation with a low P/O ratio | Starkeya novella | sulfate + ferrocytochrome c + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
complete loss of activity after 5 min, 25% loss of activity after 5 min in presence of 0.05 M sodium sulfite | Starkeya novella |
60 | - |
1 min, complete loss of activity | Starkeya novella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
potassium phosphate buffer | Starkeya novella |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
NaCl | - |
Starkeya novella |