Crystallization (Comment) | Organism |
---|---|
structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. A pocket on the surface contains a CVWC motif, identifying the active site with architecture similar to glutaredoxins. Active site modeling of coenzyme M shows the sulfate moiety hydrogen-bonded to the backbone amide and carbonyl oxygen of residue Phe 76 | Methanosarcina acetivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
reduced coenzyme M | pH 6.8, temperature not specified in the publication | Methanosarcina acetivorans | |
8.9 | - |
reduced glutathione | pH 6.8, temperature not specified in the publication | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | a cadmium ion is found within the active site of each monomer, crystallization data | Methanosarcina acetivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11500 | - |
1 * 11500, SDS-PAGE | Methanosarcina acetivorans |
12000 | - |
gel filtration | Methanosarcina acetivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | Q8TQ93 | - |
- |
Methanosarcina acetivorans DSM 2834 | Q8TQ93 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
insulin disulfide + reduced coenzyme M | - |
Methanosarcina acetivorans | insulin dithiol + oxidized coenzyme M | - |
? | |
insulin disulfide + reduced coenzyme M | - |
Methanosarcina acetivorans DSM 2834 | insulin dithiol + oxidized coenzyme M | - |
? | |
insulin disulfide + reduced glutathione | - |
Methanosarcina acetivorans | insulin dithiol + oxidized glutathione | - |
? | |
insulin disulfide + reduced glutathione | - |
Methanosarcina acetivorans DSM 2834 | insulin dithiol + oxidized glutathione | - |
? | |
additional information | no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity | Methanosarcina acetivorans | ? | - |
? | |
additional information | no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity | Methanosarcina acetivorans DSM 2834 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 11500, SDS-PAGE | Methanosarcina acetivorans |
Synonyms | Comment | Organism |
---|---|---|
MA_1658 | - |
Methanosarcina acetivorans |
methanoredoxin | - |
Methanosarcina acetivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme M | coenzyme M-SH and glutathione bind to the active site | Methanosarcina acetivorans | |
glutathione | coenzyme M-SH and glutathione bind to the active site | Methanosarcina acetivorans |