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show all sequences of 1.8.1.B5

Structural and biochemical characterizations of methanoredoxin from Methanosarcina acetivorans, a glutaredoxin-like enzyme with coenzyme M-dependent protein disulfide reductase activity

Yenugudhati, D.; Prakash, D.; Kumar, A.K.; Kumar, R.S.; Yennawar, N.H.; Yennawar, H.P.; Ferry, J.G.; Biochemistry 55, 313-321 (2016)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. A pocket on the surface contains a CVWC motif, identifying the active site with architecture similar to glutaredoxins. Active site modeling of conezyme M shows the sulfate moiety hydrogen-bonded to the backbone amide and carbonyl oxygen of residue Phe 76
Methanosarcina acetivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
reduced coenzyme M
pH 6.8, temperature not specified in the publication
Methanosarcina acetivorans
8.9
-
reduced glutathione
pH 6.8, temperature not specified in the publication
Methanosarcina acetivorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
a cadmium ion is found within the active site of each monomer, crystallization data
Methanosarcina acetivorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11500
-
1 * 11500, SDS-PAGE
Methanosarcina acetivorans
12000
-
gel filtration
Methanosarcina acetivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina acetivorans
Q8TQ93
-
-
Methanosarcina acetivorans DSM 2834
Q8TQ93
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
insulin disulfide + reduced coenzyme M
-
733364
Methanosarcina acetivorans
insulin dithiol + oxidized coenzyme M
-
-
-
?
insulin disulfide + reduced coenzyme M
-
733364
Methanosarcina acetivorans DSM 2834
insulin dithiol + oxidized coenzyme M
-
-
-
?
insulin disulfide + reduced glutathione
-
733364
Methanosarcina acetivorans
insulin dithiol + oxidized glutathione
-
-
-
?
insulin disulfide + reduced glutathione
-
733364
Methanosarcina acetivorans DSM 2834
insulin dithiol + oxidized glutathione
-
-
-
?
additional information
no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity
733364
Methanosarcina acetivorans
?
-
-
-
-
additional information
no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity
733364
Methanosarcina acetivorans DSM 2834
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 11500, SDS-PAGE
Methanosarcina acetivorans
Cofactor
Cofactor
Commentary
Organism
Structure
coenzyme M
coenzyme M-SH and glutathione bind to the active site
Methanosarcina acetivorans
glutathione
coenzyme M-SH and glutathione bind to the active site
Methanosarcina acetivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
coenzyme M
coenzyme M-SH and glutathione bind to the active site
Methanosarcina acetivorans
glutathione
coenzyme M-SH and glutathione bind to the active site
Methanosarcina acetivorans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. A pocket on the surface contains a CVWC motif, identifying the active site with architecture similar to glutaredoxins. Active site modeling of conezyme M shows the sulfate moiety hydrogen-bonded to the backbone amide and carbonyl oxygen of residue Phe 76
Methanosarcina acetivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
reduced coenzyme M
pH 6.8, temperature not specified in the publication
Methanosarcina acetivorans
8.9
-
reduced glutathione
pH 6.8, temperature not specified in the publication
Methanosarcina acetivorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
a cadmium ion is found within the active site of each monomer, crystallization data
Methanosarcina acetivorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
11500
-
1 * 11500, SDS-PAGE
Methanosarcina acetivorans
12000
-
gel filtration
Methanosarcina acetivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
insulin disulfide + reduced coenzyme M
-
733364
Methanosarcina acetivorans
insulin dithiol + oxidized coenzyme M
-
-
-
?
insulin disulfide + reduced coenzyme M
-
733364
Methanosarcina acetivorans DSM 2834
insulin dithiol + oxidized coenzyme M
-
-
-
?
insulin disulfide + reduced glutathione
-
733364
Methanosarcina acetivorans
insulin dithiol + oxidized glutathione
-
-
-
?
insulin disulfide + reduced glutathione
-
733364
Methanosarcina acetivorans DSM 2834
insulin dithiol + oxidized glutathione
-
-
-
?
additional information
no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity
733364
Methanosarcina acetivorans
?
-
-
-
-
additional information
no betahydroxyethyl disulfide reductase activity, no dehydroascorbate reductase activity
733364
Methanosarcina acetivorans DSM 2834
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 11500, SDS-PAGE
Methanosarcina acetivorans
Other publictions for EC 1.8.1.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733364
Yenugudhati
Structural and biochemical cha ...
Methanosarcina acetivorans, Methanosarcina acetivorans DSM 2834
Biochemistry
55
313-321
2016
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