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Literature summary for 1.8.1.B4 extracted from

  • Limauro, D.; Saviano, M.; Galdi, I.; Rossi, M.; Bartolucci, S.; Pedone, E.
    Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (2009), Protein Eng. Des. Sel., 22, 19-26.
    View publication on PubMed
Search for more literature for 1.8.1.B4

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00012
-
 [C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide pH 7.0, 60°C Saccharolobus solfataricus
0.00017
-
 [C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide pH 7.0, 60°C Saccharolobus solfataricus
0.0002
-
 [protein disulfide oxidoreductase]-disulfide pH 7.0, 60°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97W27
-
-
Saccharolobus solfataricus P2 Q97W27
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus [C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+
-
 ?
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus P2 [C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+
-
 ?
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus [C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+
-
 ?
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus P2 [C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+
-
 ?
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus [C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+
-
 ?
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus P2 [C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+
-
 ?
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus [C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+
-
 ?
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus P2 [C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+
-
 ?
[protein disulfide oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus [protein disulfide oxidoreductase]-dithiol + NADP+
-
 ?
[protein disulfide oxidoreductase]-disulfide + NADPH + H+ the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase Saccharolobus solfataricus P2 [protein disulfide oxidoreductase]-dithiol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
SSO2416 locus name Saccharolobus solfataricus
SsTR
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharolobus solfataricus