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Literature summary for 1.8.1.B1 extracted from

  • Bonilla, M.; Denicola, A.; Marino, S.M.; Gladyshev, V.N.; Salinas, G.
    Linked thioredoxin-glutathione systems in platyhelminth parasites: alternative pathways for glutathione reduction and deglutathionylation (2011), J. Biol. Chem., 286, 4959-4967.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.B1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Echinococcus granulosus

Protein Variants

Protein Variants Comment Organism
C31S the mutant has negligible deglutathionylase and GR activities when compared with wild type enzyme Echinococcus granulosus
C34S both the deglutathionylase and GR activities of the mutant are only slightly lower when compared with wild type enzyme Echinococcus granulosus

Inhibitors

Inhibitors Comment Organism Structure
NOC-7 i.e. 1-hydroxy-2-oxo-3-(N-3-methyl-aminopropyl)-3-methyl-1-triazene, complete inhibition at 0.1 mM Echinococcus granulosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GSSG + NADPH + H+ Echinococcus granulosus
-
 GSH + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+ Echinococcus granulosus
-
 thioredoxin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Echinococcus granulosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Echinococcus granulosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Echinococcus granulosus 2-nitro-5-thiobenzoate + NADP+
-
 ?
GSSG + NADPH + H+
-
 Echinococcus granulosus GSH + NADP+
-
 ?
thioredoxin disulfide + NADPH + H+
-
 Echinococcus granulosus thioredoxin + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
TGR
-
 Echinococcus granulosus
thioredoxin-glutathione reductase
-
 Echinococcus granulosus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Echinococcus granulosus
NADPH
-
 Echinococcus granulosus