Cloned (Comment) | Organism |
---|---|
recombinant TGR is mostly insoluble when expressed in Escherichi coli, and neither cloning of the enzyme that contain various tags nor coexpression of TGR with chaperones increases its solubility | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic properties of the Grx domain of TGR | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Danio rerio | - |
- |
- |
Gallus gallus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
no activity in Caenorhabditis elegans | - |
- |
- |
no activity in Canis familiaris | - |
- |
- |
no activity in Drosophila melanogaster | - |
- |
- |
no activity in Sus scrofa | - |
- |
- |
Pan troglodytes | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Takifugu rubripes | - |
- |
- |
Tetraodon nigroviridis | - |
- |
- |
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
small amounts of the soluble enzyme are obtained by purifying it from a large quantity of cells, in which protein synthesis is induced slowly for a period of 24 h at a low temperature | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | TGR is regulated by both selenium availability and selenocysteine tRNA status. This regulation is more pronounced in liver, whereas in testes, the expression level is only slightly affected | Mus musculus | - |
testis | TGR is regulated by both selenium availability and selenocysteine tRNA status. This regulation is more pronounced in liver, whereas in testes, the expression level is only slightly affected | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | ? | - |
? | |
glutathione disulfide + NADPH + H+ | - |
Mus musculus | glutathione + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Mus musculus | thioredoxin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TGR | - |
Gallus gallus |
TGR | - |
Mus musculus |
TGR | - |
Homo sapiens |
TGR | - |
Rattus norvegicus |
TGR | - |
Bos taurus |
TGR | - |
Xenopus laevis |
TGR | - |
Danio rerio |
TGR | - |
Pan troglodytes |
TGR | - |
Takifugu rubripes |
TGR | - |
Tetraodon nigroviridis |
thioredoxin/glutathione reductase | - |
Gallus gallus |
thioredoxin/glutathione reductase | - |
Mus musculus |
thioredoxin/glutathione reductase | - |
Homo sapiens |
thioredoxin/glutathione reductase | - |
Rattus norvegicus |
thioredoxin/glutathione reductase | - |
Bos taurus |
thioredoxin/glutathione reductase | - |
Xenopus laevis |
thioredoxin/glutathione reductase | - |
Danio rerio |
thioredoxin/glutathione reductase | - |
Pan troglodytes |
thioredoxin/glutathione reductase | - |
Takifugu rubripes |
thioredoxin/glutathione reductase | - |
Tetraodon nigroviridis |