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Literature summary for 1.8.1.B1 extracted from

  • Sun, Q.A.; Su, D.; Novoselov, S.V.; Carlson, B.A.; Hatfield, D.L.; Gladyshev, V.N.
    Reaction mechanism and regulation of mammalian thioredoxin/glutathione reductase (2005), Biochemistry, 44, 14528-14537.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant TGR is mostly insoluble when expressed in Escherichi coli, and neither cloning of the enzyme that contain various tags nor coexpression of TGR with chaperones increases its solubility Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic properties of the Grx domain of TGR Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Danio rerio
-
-
-
Gallus gallus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
no activity in Caenorhabditis elegans
-
-
-
no activity in Canis familiaris
-
-
-
no activity in Drosophila melanogaster
-
-
-
no activity in Sus scrofa
-
-
-
Pan troglodytes
-
-
-
Rattus norvegicus
-
-
-
Takifugu rubripes
-
-
-
Tetraodon nigroviridis
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
small amounts of the soluble enzyme are obtained by purifying it from a large quantity of cells, in which protein synthesis is induced slowly for a period of 24 h at a low temperature Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver TGR is regulated by both selenium availability and selenocysteine tRNA status. This regulation is more pronounced in liver, whereas in testes, the expression level is only slightly affected Mus musculus
-
testis TGR is regulated by both selenium availability and selenocysteine tRNA status. This regulation is more pronounced in liver, whereas in testes, the expression level is only slightly affected Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
Mus musculus ?
-
?
glutathione disulfide + NADPH + H+
-
Mus musculus glutathione + NADP+
-
?
thioredoxin disulfide + NADPH + H+
-
Mus musculus thioredoxin + NADP+
-
?

Synonyms

Synonyms Comment Organism
TGR
-
Gallus gallus
TGR
-
Mus musculus
TGR
-
Homo sapiens
TGR
-
Rattus norvegicus
TGR
-
Bos taurus
TGR
-
Xenopus laevis
TGR
-
Danio rerio
TGR
-
Pan troglodytes
TGR
-
Takifugu rubripes
TGR
-
Tetraodon nigroviridis
thioredoxin/glutathione reductase
-
Gallus gallus
thioredoxin/glutathione reductase
-
Mus musculus
thioredoxin/glutathione reductase
-
Homo sapiens
thioredoxin/glutathione reductase
-
Rattus norvegicus
thioredoxin/glutathione reductase
-
Bos taurus
thioredoxin/glutathione reductase
-
Xenopus laevis
thioredoxin/glutathione reductase
-
Danio rerio
thioredoxin/glutathione reductase
-
Pan troglodytes
thioredoxin/glutathione reductase
-
Takifugu rubripes
thioredoxin/glutathione reductase
-
Tetraodon nigroviridis