Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00031 | - |
thioredoxin | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
0.00235 | - |
NADPH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
0.0186 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
0.1 | - |
NADH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
x * 36500, calculated from amino acid sequence | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | Solanum lycopersicum | - |
thioredoxin disulfide + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity column chromatography | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+ | - |
Solanum lycopersicum | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Solanum lycopersicum | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
thioredoxin + NADP+ | - |
Solanum lycopersicum | thioredoxin disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36500, calculated from amino acid sequence | Solanum lycopersicum |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent thioredoxin reductase | - |
Solanum lycopersicum |
NTR | - |
Solanum lycopersicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
NADH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
0.35 | - |
NADPH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
0.38 | - |
thioredoxin | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.77 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Solanum lycopersicum | |
NADPH | the catalytic efficiency with NADPH is 2500fold higher than with NADH | Solanum lycopersicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Solanum lycopersicum | calculated from amino acid sequence | - |
5.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
NADH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
95 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
150 | - |
NADPH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1230 | - |
thioredoxin | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum |