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Literature summary for 1.8.1.9 extracted from

  • Angelucci, F.; Dimastrogiovanni, D.; Boumis, G.; Brunori, M.; Miele, A.E.; Saccoccia, F.; Bellelli, A.
    Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography (2010), J. Biol. Chem., 285, 32557-32567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Schistosoma mansoni

Crystallization (Commentary)

Crystallization (Comment) Organism
TGR alone or in complex with NADH and GSSG, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.4, 20% PEG 3350, 0.2 M KI and 5 mM beta-mercaptoethanol or 0.2 M magnesium formate, 20% PEG 6000, 0.1 M Hepes, pH 7.0-7.5 and 5 mM beta-mercaptoethanol (TGR with reduced and ordered C-terminus) Schistosoma mansoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 SDS-PAGE Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni Q962Y6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thioredoxin + NADP+
-
 Schistosoma mansoni thioredoxin disulfide + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
TGR
-
 Schistosoma mansoni
thioredoxin glutathione reductase this natural chimeric enzyme is a peculiar fusion of a glutaredoxin domain with a thioredoxin selenocysteine-containing reductase domain Schistosoma mansoni

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Schistosoma mansoni
NADP+
-
 Schistosoma mansoni