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Literature summary for 1.8.1.9 extracted from
- Structure and stability of a thioredoxin reductase from Sulfolobus solfataricus: a thermostable protein with two functions (2009), Biochim. Biophys. Acta, 1794, 554-562.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| wild type and mutant enzymes with or without NADP+, using 12-15% (w/v) polyethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4, and 50 mM sodium acetate (pH 4.6) | Saccharolobus solfataricus |
| wild type enzyme in complex with NADP+ and mutant enzyme C147A, using 12-15% (w/v) poly-ethylene glycol 2000 monomethyl ether, 0.1 M (NH4)2SO4 and 50 mM sodium acetate (pH 4.6) | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C147A | the mutant exhibits a marginal NADH oxidase activity with FAD canonically bound to the enzyme | Saccharolobus solfataricus |
| C147A | in the active site of the C147A mutant, which exhibits a marginal NADH oxidase activity, the FAD is canonically bound to the enzyme | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97W27 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| disulfide oxidase + NADPH + H+ | - |
Saccharolobus solfataricus | ? | - |
? |  |
| additional information | TrxRB3 also displays NADH oxidase activity | Saccharolobus solfataricus | ? | - |
? | |
| additional information | TrxRB3 is endowed with an additional NADH oxidase activity | Saccharolobus solfataricus | ? | - |
? | |
| thioredoxin + NADP+ | - |
Saccharolobus solfataricus | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin disulfide + NADPH + H+ | - |
Saccharolobus solfataricus | thioredoxin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | x-ray crystallography | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thioredoxin reductase | - |
Saccharolobus solfataricus |
| TrxRB3 | - |
Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Saccharolobus solfataricus | |
| NADPH | - |
Saccharolobus solfataricus | |
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Release 2023.1 (January 2023)
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