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Literature summary for 1.8.1.9 extracted from
- Selenium in thioredoxin reductase: a mechanistic perspective (2008), Biochemistry, 47, 12810-12821.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Drosophila melanogaster |
| expressed in Escherichia coli ER2566 cells | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster |  |
| 0.41 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
| 0.45 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
| 0.53 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
| 0.83 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
| 4.1 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
| mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Drosophila melanogaster | |
| selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Mus musculus | |
| selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Rattus norvegicus | |
| selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Caenorhabditis elegans | |
| selenium | the presence of a selenium atom is not chemically required to catalyze the reduction of the disulfide bond of thioredoxin | Anopheles gambiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
12% reducing SDS-PAGE | Drosophila melanogaster |
| 55000 | - |
12% reducing SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thioredoxin + NADP+ | Drosophila melanogaster | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | Mus musculus | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | Rattus norvegicus | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | Caenorhabditis elegans | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | Anopheles gambiae | - |
thioredoxin disulfide + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anopheles gambiae | - |
- |
- |
| Caenorhabditis elegans | - |
- |
- |
| Drosophila melanogaster | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography | Drosophila melanogaster |
| DEAE column column chromatography, 2',5'-ADP Sepharose 4B column chromatography, phenyl Sepharose column chromatography, and Ni-NTA agarose column chromatography | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Drosophila melanogaster | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Mus musculus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Caenorhabditis elegans | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Anopheles gambiae | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| thioredoxin + NADP+ | - |
Drosophila melanogaster | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | - |
Mus musculus | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | - |
Rattus norvegicus | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | - |
Caenorhabditis elegans | thioredoxin disulfide + NADPH + H+ | - |
? | |
| thioredoxin + NADP+ | - |
Anopheles gambiae | thioredoxin disulfide + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thioredoxin reductase | - |
Drosophila melanogaster |
| thioredoxin reductase | - |
Mus musculus |
| thioredoxin reductase | - |
Rattus norvegicus |
| thioredoxin reductase | - |
Caenorhabditis elegans |
| thioredoxin reductase | - |
Anopheles gambiae |
| TR2 | - |
Caenorhabditis elegans |
| TR3 | - |
Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.23 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
| 2.53 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
| 2.62 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
| 20.85 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
| 21.6 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
| 48.42 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
the truncated enzyme shows a pH optimum for the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at pH 6.5 | Mus musculus |
| 7.5 | - |
the truncated enzyme shows a pH optimum for the reduction of 5,5'-dithiobis(2-nitrobenzoic acid) at pH 7.5 | Drosophila melanogaster |
| 8 | 8.5 | - |
Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Drosophila melanogaster | |
| NADPH | - |
Mus musculus | |
| NADPH | - |
Rattus norvegicus | |
| NADPH | - |
Caenorhabditis elegans | |
| NADPH | - |
Anopheles gambiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
| 4.78 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
| 5.27 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Drosophila melanogaster | |
| 5.45 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Caenorhabditis elegans | |
| 45.33 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | wild type enzyme, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
| 58.33 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | truncated thioredoxin reductase missing its final eight amino acids, in 50 mM potassium phosphate at pH 7.0 | Mus musculus | |
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