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Literature summary for 1.8.1.9 extracted from
- Identification and characterization of thioredoxin and thioredoxin reductase from Aeropyrum pernix K1 (2002), Eur. J. Biochem., 269, 5423-5430.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aeropyrum pernix |
General Stability
| General Stability | Organism |
|---|---|
| FAD increases thermostability | Aeropyrum pernix |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.6 | - |
NADPH | - |
Aeropyrum pernix |  |
| 12.3 | - |
thioredoxin | - |
Aeropyrum pernix | |
| 172.4 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aeropyrum pernix | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Aeropyrum pernix |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | - |
K1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Aeropyrum pernix | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
| thioredoxin disulfide + NADPH | - |
Aeropyrum pernix | thioredoxin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Aeropyrum pernix |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ApTR | - |
Aeropyrum pernix |
| thioredoxin reductase | - |
Aeropyrum pernix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
above | Aeropyrum pernix |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | 70°C: about 50% of maximal activity, 95°C: about 95% of maximal activity | Aeropyrum pernix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 110 | - |
half-life: 4 min | Aeropyrum pernix |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aeropyrum pernix | |
| 63.2 | - |
thioredoxin | - |
Aeropyrum pernix | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Aeropyrum pernix | |
| NADPH | - |
Aeropyrum pernix | |
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