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Literature summary for 1.8.1.9 extracted from

  • Gorlatov, S.N.; Stadtman, T.C.
    Human selenium-dependent thioredoxin reductase from HeLa cells: properties of forms with differing heparin affinities (1999), Arch. Biochem. Biophys., 369, 133-142.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate under growth conditions Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoform TrxR1 Homo sapiens 5829
-
mitochondrion isoform TrxR2 Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
selenocysteine heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine Homo sapiens
selenocysteine contains selenocysteine as part of the redox active selenosulfide in the active center Homo sapiens
selenocysteine isoform 1 contains 1 selenium per subunit, the mitochondrial isoform 2 contains 0.5 selenium per subunit Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54800
-
2 * 54800, HeLa cell enzyme, MALDI Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
HeLa cells
-

Oxidation Stability

Oxidation Stability Organism
the reduced enzyme is very labile to O2, decrease of selenium content and activity after exposure to air, protection by NADPH, NADP+ and tris-(2-carboxyethyl)phosphine Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ conformational change upon reduction wth NADPH or tris-(2-carboxyethyl)phosphine Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell high and low affinity form with respect to heparin Homo sapiens
-
HeLa cell heparin affinity depends on the selenium content Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 54800, HeLa cell enzyme, MALDI Homo sapiens

Synonyms

Synonyms Comment Organism
TrxR
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens