Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-chloro-2,4-dinitrobenzene | i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH | Bos taurus | |
1-chloro-2,4-dinitrobenzene | i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH | Homo sapiens | |
1-Fluoro-2,4-dinitrobenzene | irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH | Bos taurus | |
1-Fluoro-2,4-dinitrobenzene | irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH | Homo sapiens | |
4-Vinylpyridine | irreversible | Bos taurus | |
4-Vinylpyridine | irreversible | Homo sapiens | |
iodoacetate | irreversible | Bos taurus | |
iodoacetate | irreversible | Homo sapiens | |
additional information | no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene | Bos taurus | |
additional information | no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
- |
Homo sapiens |
120000 | - |
- |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
thymus | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Bos taurus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Bos taurus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Bos taurus | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Bos taurus | thioredoxin disulfide + NADPH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Homo sapiens |
20 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
8 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens | |
NADPH | - |
Bos taurus |