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Literature summary for 1.8.1.9 extracted from

  • Nordberg, J.; Zhong, L.; Holmgren, A.; Arner, E.S.J.
    Mammalian thioredoxin reductase is irreversibly inhibited by dinitrohalobenzenes by alkylation of both the redox active selenocysteine and its neighboring cysteine residue (1998), J. Biol. Chem., 273, 10835-10842.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-2,4-dinitrobenzene i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH Bos taurus
1-chloro-2,4-dinitrobenzene i.e. DNCB; irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH Homo sapiens
1-Fluoro-2,4-dinitrobenzene irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH Bos taurus
1-Fluoro-2,4-dinitrobenzene irreversible, with NADPH, alkylation of the active site cysteine disulfide, strong increase in oxidation activity of the enzyme against NADPH Homo sapiens
4-Vinylpyridine irreversible Bos taurus
4-Vinylpyridine irreversible Homo sapiens
iodoacetate irreversible Bos taurus
iodoacetate irreversible Homo sapiens
additional information no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene Bos taurus
additional information no inhibition with dinitrohalobenzene analogues: 1,4-dichlorobenzene, 1-chloro-4-nitrobenzene, 1-chloro-3,4-dinitrobenzene, 1-chloro-2,5-dinitrobenzene Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
-
Homo sapiens
120000
-
-
Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Bos taurus 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Bos taurus 2-nitro-5-thiobenzoate + NADP+
-
?
thioredoxin + NADP+ coupled assay with DTNB Homo sapiens thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay with DTNB Bos taurus thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Homo sapiens thioredoxin disulfide + NADPH
-
?
thioredoxin + NADP+ coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin Bos taurus thioredoxin disulfide + NADPH
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Homo sapiens
20
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens
8
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens
NADPH
-
Homo sapiens
NADPH
-
Bos taurus