General Stability | Organism |
---|---|
tightly bound FAD is not replaced during heat treatment or guanidinium hydrochloride | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Homo sapiens |
130000 | 160000 | sucrose density gradient centrifugation and gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | Homo sapiens | - |
thioredoxin disulfide + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | three-dimensional structure | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.8 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Homo sapiens | ? | - |
? | |
thioredoxin + NADP+ | - |
Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Homo sapiens | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | in presence of NADPH, coupled assay | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | i.e. DTNB | Homo sapiens | thioredoxin disulfide + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
reduction of insulin | Homo sapiens |
52 | - |
reduction of DTNB | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, no loss of activity | Homo sapiens |
65 | - |
above, irreversible loss of activity, no displacement of tightly bound FAD | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |