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Literature summary for 1.8.1.9 extracted from
- An NADP/thioredoxin system in leaves: purification and characterization of NADP-thioredoxin reductase and thioredoxin h from spinach (1988), Arch. Biochem. Biophys., 266, 496-507.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
NADPH | - |
Spinacia oleracea |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
2 * 33000, SDS-PAGE | Spinacia oleracea |
| 68000 | - |
gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Spinacia oleracea |
| 20 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dithiothreitol + NADPH | - |
Spinacia oleracea | ? | - |
? | |
| thioredoxin + NADP+ | coupled assay with dithiothreitol | Spinacia oleracea | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | coupled assay with DTNB | Spinacia oleracea | thioredoxin disulfide + NADPH | - |
? | |
| thioredoxin + NADP+ | i.e. DTNB | Spinacia oleracea | thioredoxin disulfide + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 33000, SDS-PAGE | Spinacia oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTR | - |
Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2 FAD per dimer | Spinacia oleracea | |
| NADP+ | - |
Spinacia oleracea | |
| NADPH | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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