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Literature summary for 1.8.1.9 extracted from
- Purification of thioredoxin, thioredoxin reductase, and glutathione reductase by affinity chromatography (1977), J. Biol. Chem., 252, 6367-6372.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | oxidation-reduction cycle of thioredoxin | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67 | - |
purified enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thioredoxin + NADP+ | - |
Escherichia coli | thioredoxin disulfide + NADPH | - |
ir |  |
| thioredoxin + NADP+ | - |
Escherichia coli 11 | thioredoxin disulfide + NADPH | - |
ir | |
| thioredoxin + NADP+ | in presence of NADPH, coupled assay | Escherichia coli | thioredoxin disulfide + NADPH + H+ | - |
ir | |
| thioredoxin + NADP+ | i.e. DTNB | Escherichia coli | thioredoxin disulfide + NADPH + H+ | - |
ir | |
| thioredoxin + NADP+ | in presence of NADPH, coupled assay | Escherichia coli 11 | thioredoxin disulfide + NADPH + H+ | - |
ir | |
| thioredoxin + NADP+ | i.e. DTNB | Escherichia coli 11 | thioredoxin disulfide + NADPH + H+ | - |
ir | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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