Literature summary for 1.8.1.8 extracted from

Pedone, E.; Saviano, M.; Bartolucci, S.; Rossi, M.; Ausili, A.; Scirè, A.; Bertoli, E.; Tanfani, F.
Temperature-, SDS-, and pH-induced conformational changes in protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A dynamic simulation and fourier transform infrared spectroscopic study (2005), J. Proteome Res., 4, 1972-1980 .

General Stability

General Stability	Organism
The structure and thermal stability of the protein are analyzed under different conditions by Fourier transform infrared spectroscopy and Molecular Dynamics simulations. The effects of SDS, pH, and temperature on the protein structure are characterized. The data indicated that pD affects differently the thermostability of alpha-helices and beta-sheets, and that 0.5% or higher SDS concentration have a significant repercussion on the structure	Pyrococcus furiosus

Organism

The structure and thermal stability of the protein are analyzed under different conditions by Fourier transform infrared spectroscopy and Molecular Dynamics simulations. The effects of SDS, pH, and temperature on the protein structure are characterized. The data indicated that pD affects differently the thermostability of alpha-helices and beta-sheets, and that 0.5% or higher SDS concentration have a significant repercussion on the structure

Pyrococcus furiosus

Organic Solvent Stability

Organic Solvent	Comment	Organism
SDS	The secondary structure of the protein is not affected by 0.1 or 0.2% of SDS. In the presence of 0.5%, the intensity of the alpha-helix band decreases markedly while the intensity of the beta-sheet band is slightly affected. In the presence of 0.7% SDS the beta-sheet band intensity decreases further and in the presence of 4% SDS it results very low	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	computational analysis reveals the different behavior of the two active sites	Pyrococcus furiosus	?	-	?

Synonyms

Synonyms	Comment	Organism
PfPDO	-	Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	pH affects differently the thermostability of alpha-helices and beta-sheets	Pyrococcus furiosus
20	-	the C terminal active site at 20°C shows a reduced flexibility at all pH values, while at 100°C the flexibility increases with the pH, with a high value at pH 7.0	Pyrococcus furiosus
100	-	flexibility increases with the pH, with a high value at pH 7.0	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Pyrococcus furiosus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.8	-	the residual amide II band intensity is higher at pH 5.8 than at pH 7.0 suggesting that the acidic pH induces a more compact structure At pH 5.8, pH not optimum for the activity, and at 10°C and 100°C, the alpha2 helix region, where the N-terminal active site is localized, is less flexible than at pH 7.0 and pH 10.0	Pyrococcus furiosus
7	-	the residual amide II band intensity is higher at pH 5.8 than at pH 7.0 suggesting that the acidic pH induces a more compact structure. At pH 5.8, pH not optimum for the activity, and at 10°C and 100°C, the alpha2 helix region, where the N-terminal active site is localized, is less flexible than at pH 7.0 and pH 10.0	Pyrococcus furiosus
10	-	at pH 10.0 the protein undergoes partial unfolding. Decrease in alpha-helix content at pH 10.0, whereas the beta-sheet content remains unaltered. At pH 5.8, pH not optimum for the activity, and at 10°C and 100°C, the alpha2 helix region, where the N-terminal active site is localized, is less flexible than at pH 7.0 and pH 10.0. The C terminal active site at 20°C shows a reduced flexibility at all pH values, while at 100°C the flexibility increases with the pH, with a high value at pH 7.0	Pyrococcus furiosus