Literature summary for 1.8.1.8 extracted from

Yenugudhati, D.; Prakash, D.; Kumar, A.K.; Kumar, R.S.; Yennawar, N.H.; Yennawar, H.P.; Ferry, J.G.
Structural and biochemical characterizations of methanoredoxin from Methanosarcina acetivorans, a glutaredoxin-like enzyme with coenzyme M-dependent protein disulfide reductase activity (2016), Biochemistry, 55, 313-321 .

Cloned(Commentary)

Cloned (Comment)	Organism
-	Methanosarcina acetivorans

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. The crystal lattice has four monomers in a dimer of dimers arrangement. A cadmium ion is found within the active site of each monomer, and two cadmium ions stabilize the N-terminal tails and dimer interfaces. CoMSH and glutathione bind to the active site of methanoredoxin similar to the binding of glutathione in glutaredoxin	Methanosarcina acetivorans

Crystallization (Comment)

Organism

crystal structure exhibits a classic thioredoxin-glutaredoxin fold comprising three alpha-helices surrounding four antiparallel beta-sheets. The crystal lattice has four monomers in a dimer of dimers arrangement. A cadmium ion is found within the active site of each monomer, and two cadmium ions stabilize the N-terminal tails and dimer interfaces. CoMSH and glutathione bind to the active site of methanoredoxin similar to the binding of glutathione in glutaredoxin

Methanosarcina acetivorans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
2.2	-	coenzyme M	pH 6.8, 50°C	Methanosarcina acetivorans
4.1	-	coenzyme A	pH 6.8, 50°C	Methanosarcina acetivorans
8.9	-	glutathione disulfide	pH 6.8, 50°C	Methanosarcina acetivorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
12000	-	gel filtration	Methanosarcina acetivorans

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina acetivorans	Q8TQ93	-	-
Methanosarcina acetivorans DSM 2834	Q8TQ93	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
coenzyme M disulfide + 5,5'-dithiobis(2-nitrobenzoic acid)	-	Methanosarcina acetivorans	2 coenzyme M + thionitrobenzoate	-	?
coenzyme M disulfide + 5,5'-dithiobis(2-nitrobenzoic acid)	-	Methanosarcina acetivorans DSM 2834	2 coenzyme M + thionitrobenzoate	-	?
coenzyme M disulfide + glutathione disulfide	-	Methanosarcina acetivorans	2 coenzyme M + glutathione	-	?
coenzyme M disulfide + glutathione disulfide	-	Methanosarcina acetivorans DSM 2834	2 coenzyme M + glutathione	-	?
insulin disulfide + 2 coenzyme A	-	Methanosarcina acetivorans	insulin + coenzyme A disulfide	-	?
insulin disulfide + 2 coenzyme A	-	Methanosarcina acetivorans DSM 2834	insulin + coenzyme A disulfide	-	?
insulin disulfide + 2 coenzyme M	-	Methanosarcina acetivorans	insulin + coenzyme M disulfide	-	?
insulin disulfide + 2 coenzyme M	-	Methanosarcina acetivorans DSM 2834	insulin + coenzyme M disulfide	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 11500, calculated and SDS-PAGE	Methanosarcina acetivorans

Synonyms

Synonyms	Comment	Organism
glutaredoxin	-	Methanosarcina acetivorans
MA_1658	-	Methanosarcina acetivorans
methanoredoxin	-	Methanosarcina acetivorans

Cofactor

Cofactor	Comment	Organism	Structure
coenzyme M	-	Methanosarcina acetivorans
additional information	no cofactor: coenzyme B	Methanosarcina acetivorans