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Literature summary for 1.8.1.8 extracted from
- Protein disulfide oxidoreductase from Pyrococcus furiosus: biochemical properties (2001), Methods Enzymol., 334, 62-73.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25649 | - |
x * 25649, electrospray mass spectrometry | Pyrococcus furiosus |
| 26000 | - |
x * 26000, SDS-PAGE | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable fo at least 3 months without any loss of activity | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin disulfide + NADPH | - |
Pyrococcus furiosus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Pyrococcus furiosus |
| ? | x * 25649, electrospray mass spectrometry | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
3 h, no loss of activity | Pyrococcus furiosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | isoelectric focusing | - |
4.9 |
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