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Literature summary for 1.8.1.8 extracted from

  • Garg, S.K.; Suhail Alam, M.; Soni, V.; Radha Kishan, K.V.; Agrawal, P.
    Characterization of Mycobacterium tuberculosis WhiB1/Rv3219 as a protein disulfide reductase (2007), Protein Expr. Purif., 52, 422-432.
    View publication on PubMed
Search for more literature for 1.8.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 2 * 14000, SDS-PAGE Mycobacterium tuberculosis
29000
-
 gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis Rv3219
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cell extract: 1.56 U/mg, after purification: 8.17 U/mg, one unit is defined as the amount of protein that produces an increase in 0.1 OD in 1 min at 650 nm Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
insulin disulfide + ?
-
 Mycobacterium tuberculosis ?
-
 ?
insulin disulfide + ?
-
 Mycobacterium tuberculosis Rv3219 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 14000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
protein disulfide reductase
-
 Mycobacterium tuberculosis
WhiB1 wild type enzyme Mycobacterium tuberculosis
WhiB1/Rv3219 recombinant enzyme Mycobacterium tuberculosis