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Literature summary for 1.8.1.8 extracted from

  • Hatch, M.D.; Turner, J.F.
    A protein disulfide reductase from pea seeds (1960), Biochem. J., 76, 556-562.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.8

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.009 mM, 66% inhibition Pisum sativum
arsenite 0.15 mM, complete inhibition Pisum sativum
CdSO4 0.005 mM, 91% inhibition Pisum sativum
CuSO4 0.015 mM, complete inhibition Pisum sativum
HgCl2 0.002 mM, 92% inhibition Pisum sativum
iodoacetate 0.054 mM, 57% inhibition Pisum sativum
p-chloromercuribenzoate 0.054 mM, 88% inhibition Pisum sativum
ZnSO4 0.003 mM, 82% inhibition Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 NADPH
-
 Pisum sativum

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-
Hordeum vulgare
-
-
-
Pisum sativum
-
-
-
Triticum aestivum
-
-
-
Vicia faba
-
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Hordeum vulgare
-
root
-
 Pisum sativum
-
root
-
 Zea mays
-
seed
-
 Vicia faba
-
seed
-
 Triticum aestivum
-
seed
-
 Hordeum vulgare
-
seed
-
 Pisum sativum
-
seed
-
 Zea mays
-
seed
-
 Avena sativa
-
shoot
-
 Vicia faba
-
shoot
-
 Triticum aestivum
-
shoot
-
 Hordeum vulgare
-
shoot
-
 Pisum sativum
-
shoot
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pisum sativum

Storage Stability

Storage Stability Organism
-15°C, 8 weeks, stable Vicia faba
-15°C, 8 weeks, stable Triticum aestivum
-15°C, 8 weeks, stable Hordeum vulgare
-15°C, 8 weeks, stable Pisum sativum
-15°C, 8 weeks, stable Zea mays
-15°C, 8 weeks, stable Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + protein disulfide
-
 Vicia faba NAD(P)+ + protein dithiol
-
 ?
NAD(P)H + protein disulfide
-
 Triticum aestivum NAD(P)+ + protein dithiol
-
 ?
NAD(P)H + protein disulfide
-
 Hordeum vulgare NAD(P)+ + protein dithiol
-
 ?
NAD(P)H + protein disulfide
-
 Zea mays NAD(P)+ + protein dithiol
-
 ?
NAD(P)H + protein disulfide
-
 Avena sativa NAD(P)+ + protein dithiol
-
 ?
NAD(P)H + protein disulfide reaction rate with NADPH is approximately twice that with NADH Pisum sativum NAD(P)+ + protein dithiol
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 10 min, stable Pisum sativum
70
-
 10 min, complete inactivation Pisum sativum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7.4
-
 Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.8 pH 6.2: about 50% of activity maximum, pH 7.8: about 20% of activity maximum Pisum sativum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 5°C or 22°C, irreversible inactivation Pisum sativum

Cofactor

Cofactor Comment Organism Structure
NADH reaction rate with NADPH is approximately twice of that with NADH Pisum sativum
NADPH reaction rate with NADPH is approximately twice of that with NADH Pisum sativum