Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Xanthomonas campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0526 | - |
NADPH | - |
Xanthomonas campestris | |
0.0555 | - |
NADH | - |
Xanthomonas campestris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | Q56S00 | Xanthomonas campestris pv. phaseoli | - |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Xanthomonas campestris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.039 | - |
using NADH as electron donor | Xanthomonas campestris |
0.045 | - |
using NADPH as electron donor | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADH + H+ | - |
Xanthomonas campestris | 2 glutathione + NAD+ | - |
? | |
glutathione disulfide + NADH + H+ | - |
Xanthomonas campestris | glutathione + NAD+ | - |
? | |
glutathione disulfide + NADPH + H+ | - |
Xanthomonas campestris | glutathione + NADP+ | - |
r | |
additional information | the enzyme has the ability to utilize both NADPH and NADH as electron donors | Xanthomonas campestris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, gel filtration | Xanthomonas campestris |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Xanthomonas campestris |
Gor | - |
Xanthomonas campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32.5 | - |
NADH | - |
Xanthomonas campestris | |
37.5 | - |
NADPH | - |
Xanthomonas campestris |