Any feedback?
Literature summary for 1.8.1.7 extracted from
- Biochemical and molecular characterisation of wheat chloroplastic glutathione reductase (2001), Biol. Plant., 44, 509-516.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 isoforms: DNA sequence analysis, multiple gene copies | Triticum aestivum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl dicarbonate | chloroplast enzyme, 100% inhibition at 4 mM, 23% inhibition at 1 mM | Triticum aestivum |  |
| N-ethylmaleimide | chloroplast enzyme, 90% inhibition at 1 mM, partially reversible by GSSG 0.5 mM | Triticum aestivum | |
| NADPH | chloroplast enzyme, 50% inhibition at 0.1 mM | Triticum aestivum | |
| p-chloromercuribenzoate | chloroplast enzyme, 96% inhibition at 1 mM | Triticum aestivum | |
| Phenylmethylsulfonylfluoride | i.e. PMSF, chloroplast enzyme, slight inhibition | Triticum aestivum | |
| Zn2+ | chloroplast enzyme, 94% inhibition at 0.5 mM, reversible and competitive to GSSG | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADPH | chloroplast enzyme | Triticum aestivum | |
| 0.04 | - |
GSSG | chloroplast enzyme | Triticum aestivum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | stroma | Triticum aestivum | 9507 | - |
| chloroplast | isoform dependent | Triticum aestivum | 9507 | - |
| cytoplasm | isoform dependent | Triticum aestivum | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 56000 + associated polypeptide 1 * 32000, SDS-PAGE | Triticum aestivum |
| 56000 | - |
2 * 56000 + associated polypeptide 1 * 32000, SDS-PAGE | Triticum aestivum |
| 150000 | - |
chloroplast enzyme, gel filtration | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
2 charge- and mass-separable isoforms in chloroplasts and cytosol | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chloroplast enzyme | Triticum aestivum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | sulfhydryl groups and histidine residues are essential for the catalytic properties of the enzyme | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 96 | - |
purified enzyme | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | highly specific for GSSG | Triticum aestivum | glutathione + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | 2 * 56000 + associated polypeptide 1 * 32000, SDS-PAGE | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GR | - |
Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
exponential increase in activity up to 45°C | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
NADPH + GSSG | Triticum aestivum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
