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Literature summary for 1.8.1.7 extracted from
- Purification by affinity chromatography of glutathione reductase (EC 1.6.4.2) from Escherichia coli and characterization of such enzyme (1984), Z. Naturforsch. C, 39, 908-915.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes | Escherichia coli |
| FAD, stabilizes | Escherichia coli |
| glycerol stabilizes | Escherichia coli |
| NADP+ stabilizes | Escherichia coli |
| pure enzyme heated in presence of bovine serum albumin and 0.020 mM NADPH: loss of activity beyond 30°C, significant reduction even at 0°C | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli | |
| 0.016 | - |
NADPH | - |
Escherichia coli |  |
| 0.066 | - |
GSSG | - |
Escherichia coli | |
| 0.377 | - |
NADH | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000 | Escherichia coli |
| 109000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | Escherichia coli | - |
glutathione + NADP+ | - |
r | |
| GSSG + NADPH | Escherichia coli S33 | - |
glutathione + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli S33 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 361 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, in the dark, 10% glycerol, 5 mM NADP+, for at least 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADH + H+ | - |
Escherichia coli | glutathione + NAD+ | - |
? | |
| glutathione disulfide + NADH + H+ | - |
Escherichia coli S33 | glutathione + NAD+ | - |
? | |
| GSSG + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | - |
Escherichia coli S33 | glutathione + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000 | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
highly stable, significant loss of activity only after 10 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
NADH + GSSG | Escherichia coli |
| 7.5 | - |
NADPH + GSSG | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
unstable below | Escherichia coli |
| 7.5 | 9.5 | 4°C, stable | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | flavoprotein | Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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