General Stability | Organism |
---|---|
repeated freezing and thawing, stable | Oryctolagus cuniculus |
urea, quite stable to | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
NADPH | - |
Oryctolagus cuniculus | |
0.058 | - |
GSSG | - |
Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE, gel filtration, monomer, enzyme can also be active as dimer | Oryctolagus cuniculus |
108000 | - |
ultracentrifugation in glycerol density gradient, dimer, enzyme can also be active also as monomer | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, with several freeze-thawing cycles, more than 2 years, stable | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | - |
Oryctolagus cuniculus | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, SDS-PAGE, enzyme can also be active as monomer | Oryctolagus cuniculus |
monomer | 1 * 56000, SDS-PAGE, enzyme can also be active as dimer | Oryctolagus cuniculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
1 h, 25% loss of activity | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 9 | at pH 5.2 and 9.0 about 20% of activity maximum | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD enzyme | Oryctolagus cuniculus | |
NADPH | absolute specificity for NADPH | Oryctolagus cuniculus |