Crystallization (Comment) | Organism |
---|---|
hanging-drop method at 22°C, size of crystals varies with ammonium sulfate concentration | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADPH | - |
Sus scrofa | |
0.076 | - |
GSSG | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, SDS-PAGE | Sus scrofa |
103000 | - |
analytical ultracentrifugation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
221 | - |
purified enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | - |
Sus scrofa | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59000, SDS-PAGE | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
NADH + GSSG | Sus scrofa |
7 | - |
NADPH + GSSG | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol of FAD per mol of enzyme | Sus scrofa | |
FAD | FAD enzyme | Sus scrofa | |
NADH | activity relative to NADPH: 0.5% | Sus scrofa | |
NADPH | best electron donor | Sus scrofa |