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Literature summary for 1.8.1.7 extracted from
- Flavin-containing dehydrogenases (1976), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 13, 89-173.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | no inhibition | Homo sapiens |  |
| glutathione | product inhibition | Homo sapiens | |
| N-ethylmaleimide | - |
Saccharomyces cerevisiae | |
| NADP+ | - |
Homo sapiens | |
| NADP+ | - |
Rattus norvegicus | |
| NADP+ | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Homo sapiens | |
| p-chloromercuriphenyl sulfonate | - |
Saccharomyces cerevisiae | |
| phenyl mercuric acetate | - |
Saccharomyces cerevisiae | |
| phosphate buffer | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
NADPH | - |
Saccharomyces cerevisiae | |
| 0.0095 | - |
NADPH | 0.03 M phosphate buffer | Homo sapiens | |
| 0.013 | - |
NADPH | 0.3 M phosphate buffer | Homo sapiens | |
| 0.019 | - |
GSSG | 0.03 M phosphate buffer | Homo sapiens | |
| 0.055 | - |
GSSG | - |
Saccharomyces cerevisiae | |
| 0.125 | - |
GSSG | 0.3 M phosphate buffer | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | cytoplasm-like spaces of mitochondria | Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | Homo sapiens | - |
glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Saccharomyces cerevisiae | - |
glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Hemicentrotus pulcherrimus | - |
glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Escherichia coli | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Rattus norvegicus | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Rattus norvegicus | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
ir | |
| GSSG + NADPH | Penicillium chrysogenum | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Escherichia coli B / ATCC 11303 | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Escherichia coli Crookes | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | Escherichia coli M191-6 | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | glutathione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
review | - |
| Hemicentrotus pulcherrimus | - |
sea urchin, review | - |
| Homo sapiens | - |
- |
- |
| Penicillium chrysogenum | - |
review | - |
| Rattus norvegicus | - |
review | - |
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | - |
Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | - |
Hemicentrotus pulcherrimus | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Rattus norvegicus | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Rattus norvegicus | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Saccharomyces cerevisiae | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Saccharomyces cerevisiae | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Penicillium chrysogenum | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Penicillium chrysogenum | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Hemicentrotus pulcherrimus | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Rattus norvegicus | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Rattus norvegicus | glutathione + NADP+ | - |
ir | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Penicillium chrysogenum | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli B / ATCC 11303 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli B / ATCC 11303 | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli B / ATCC 11303 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli Crookes | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli Crookes | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli Crookes | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli M191-6 | glutathione + NADP+ | - |
? | |
| GSSG + NADPH | glutathione or other disulfides, e.g.: bis-L-gamma-glutamyl-L-cystinyl-bis-beta-alanine | Escherichia coli M191-6 | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | production of substrate for: glutathione peroxidase EC 1.11.1.9, glutathione-homocystine oxidoreductase EC 1.8.4.1, glutathione-protein disulfide oxidoreductase EC 1.8.4.2 | Escherichia coli M191-6 | glutathione + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
| 25 | - |
assay at | Homo sapiens |
| 25 | - |
assay at | Saccharomyces cerevisiae |
| 25 | - |
assay at | Hemicentrotus pulcherrimus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48.3 | - |
glutathione disulfide | reoxidation | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
broad optimum | Homo sapiens |
| 7 | - |
NADPH + GSSG | Rattus norvegicus |
| 7 | - |
NADPH + GSSG | Saccharomyces cerevisiae |
| 8 | - |
glutathione disulfide oxidation | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| FAD | - |
Rattus norvegicus | |
| FAD | - |
Penicillium chrysogenum | |
| FAD | - |
Hemicentrotus pulcherrimus | |
| FAD | 5 thiol groups per FAD | Escherichia coli | |
| FAD | 4 thiol groups per FAD | Saccharomyces cerevisiae | |
| NADPH | - |
Escherichia coli | |
| NADPH | - |
Homo sapiens | |
| NADPH | - |
Rattus norvegicus | |
| NADPH | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Penicillium chrysogenum | |
| NADPH | - |
Hemicentrotus pulcherrimus | |
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