Literature summary for 1.8.1.5 extracted from

Prussia, G.A.; Shisler, K.A.; Zadvornyy, O.A.; Streit, B.R.; DuBois, J.L.; Peters, J.W.
The unique Phe-His dyad of 2-ketopropyl coenzyme M oxidoreductase/carboxylase selectively promotes carboxylation and S-C bond cleavage (2021), J. Biol. Chem., 297, 100961 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS or BL21(DE3) cells	Xanthobacter autotrophicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using 0.17 M ammonium acetate, 0.085 M trisodium citrate, pH 5.6, 27% (w/v) PEG 4000, and 15% (v/v) glycerol	Xanthobacter autotrophicus

Protein Variants

Protein Variants	Comment	Organism
F501H	the mutant shows 10% acetoacetate production activity compared to the wild type enzyme. The overall rate of NADPH turnover remains relatively unchanged in the F501H variant relative to wild type. Moreover, acetone formation by F501H is comparable in rate to the carboxylation reaction catalyzed by wild type enzyme and leading to acetoacetate	Xanthobacter autotrophicus
F501H/H506E	inactive	Xanthobacter autotrophicus
H506E	the mutant shows 37% acetoacetate production activity compared to the wild type enzyme. NADPH turnover is around 1.5fold slower in H506E versus wild type enzyme	Xanthobacter autotrophicus

Inhibitors

Inhibitors	Comment	Organism	Structure
acetoacetate	mixed noncompetitive inhibition	Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-oxopropyl-coenzyme M + CO2 + NADPH	Xanthobacter autotrophicus	-	coenzyme M + acetoacetate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthobacter autotrophicus	Q56839	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxopropyl-coenzyme M + CO2 + NADPH	-	Xanthobacter autotrophicus	coenzyme M + acetoacetate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
2-ketopropyl coenzyme M oxidoreductase/carboxylase	-	Xanthobacter autotrophicus
2-KPCC	-	Xanthobacter autotrophicus

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Release 2023.1 (January 2023)