Application | Comment | Organism |
---|---|---|
additional information | the lipoyl protein domain (but not lipoic acid alone) plays a regulatory role in the enzymatic characteristics of pneumococcal DLDH | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
K43A | unable to express the mutant protein | Streptococcus pneumoniae |
K43R | expresses well, but still has lipoic acid attached | Streptococcus pneumoniae |
additional information | protein expressed without lipoic acid is indistinguishable from the wild-type protein. The protein without a lipoyl protein domain has a 2-3fold higher turnover number, a lower Ki for NADH, and a higher Ki for lipoamide compared with the other two enzymes | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Lipoamide | - |
Streptococcus pneumoniae | |
NADH | - |
Streptococcus pneumoniae | |
potassium phosphate | when purified DLDH is eluted directly into potassium phosphate buffer, the enzymatic activity rapidly decreases | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
dihydrolipoamide | recombinant DLDH expressed without without a lipoyl protein domain | Streptococcus pneumoniae | |
0.056 | - |
dihydrolipoamide | recombinant DLDH | Streptococcus pneumoniae | |
0.064 | - |
dihydrolipoamide | recombinant DLDH expressed without lipoic acid | Streptococcus pneumoniae | |
0.82 | - |
NAD+ | recombinant DLDH expressed without without a lipoyl protein domain | Streptococcus pneumoniae | |
0.985 | - |
NAD+ | recombinant DLDH expressed without lipoic acid | Streptococcus pneumoniae | |
1.037 | - |
NAD+ | recombinant DLDH | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61600 | - |
full-length recombinant DLDH, sequence analysis | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, at least 90% pure | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Streptococcus pneumoniae | lipoamide + NADH | - |
r | |
lipoamide + NADH | - |
Streptococcus pneumoniae | dihydrolipoamide + NAD+ | - |
r | |
nitro blue tetrazolium + NADH | - |
Streptococcus pneumoniae | ? + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Streptococcus pneumoniae |
DLDH | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
NAD+ | recombinant DLDH | Streptococcus pneumoniae | |
223 | - |
NAD+ | recombinant DLDH expressed without lipoic acid | Streptococcus pneumoniae | |
506 | - |
NAD+ | recombinant DLDH expressed without without a lipoyl protein domain | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
in the reverse reaction | Streptococcus pneumoniae |
8.5 | - |
in the forward reaction | Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Streptococcus pneumoniae | |
additional information | unable to use NADP+ and NADPH | Streptococcus pneumoniae | |
NAD+ | - |
Streptococcus pneumoniae | |
NADH | - |
Streptococcus pneumoniae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
NADH | recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 1.5 mM dihydrolipoamide | Streptococcus pneumoniae | |
0.0107 | - |
NADH | recombinant DLDH expressed without lipoic acid, in the presence of 1.5 mM dihydrolipoamide | Streptococcus pneumoniae | |
0.0149 | - |
NADH | recombinant DLDH, in the presence of 1.5 mM dihydrolipoamide | Streptococcus pneumoniae | |
0.0351 | - |
NADH | recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 2 mM NAD+ | Streptococcus pneumoniae | |
0.0488 | - |
Lipoamide | recombinant DLDH expressed without lipoic acid, in the presence of 4 mM NAD+ | Streptococcus pneumoniae | |
0.0566 | - |
Lipoamide | recombinant DLDH, in the presence of 4 mM NAD+ | Streptococcus pneumoniae | |
0.0722 | - |
NADH | recombinant DLDH, in the presence of 2 mM NAD+ | Streptococcus pneumoniae | |
0.084 | - |
NADH | recombinant DLDH expressed without lipoic acid, in the presence of 2 mM NAD+ | Streptococcus pneumoniae | |
0.1899 | - |
Lipoamide | recombinant DLDH expressed without lipoic acid, in the presence of 1.5 mM dihydrolipoamide | Streptococcus pneumoniae | |
0.1953 | - |
Lipoamide | recombinant DLDH, in the presence of 1.5 mM dihydrolipoamide | Streptococcus pneumoniae | |
0.349 | - |
Lipoamide | recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 4 mM NAD+ | Streptococcus pneumoniae | |
0.8793 | - |
Lipoamide | recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 1.5 mM dihydrolipoamide | Streptococcus pneumoniae |