Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D5A | 4.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
E91A | 49.6% activity of the wild type enzyme | Mycobacterium tuberculosis |
E91K | 62.5% activity of the wild type enzyme | Mycobacterium tuberculosis |
F464A | 5% activity of the wild type enzyme | Mycobacterium tuberculosis |
H386A | 20% activity of the wild type enzyme | Mycobacterium tuberculosis |
H386K | 9% activity of the wild type enzyme | Mycobacterium tuberculosis |
H460E | 69.4% activity of the wild type enzyme | Mycobacterium tuberculosis |
H98A | 3.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
K103E | 17.7% activity of the wild type enzyme | Mycobacterium tuberculosis |
K105A | 44.7% activity of the wild type enzyme | Mycobacterium tuberculosis |
K216A | 58.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
K220A | 81.2% activity of the wild type enzyme | Mycobacterium tuberculosis |
K223A | 67.1% activity of the wild type enzyme | Mycobacterium tuberculosis |
K223E | 70.5% activity of the wild type enzyme | Mycobacterium tuberculosis |
K224A | 55.2% activity of the wild type enzyme | Mycobacterium tuberculosis |
K376A | 52.2% activity of the wild type enzyme | Mycobacterium tuberculosis |
K67A | 67.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
K67E | 64.3% activity of the wild type enzyme | Mycobacterium tuberculosis |
K88E | 74.8% activity of the wild type enzyme | Mycobacterium tuberculosis |
N43A | 11% activity of the wild type enzyme | Mycobacterium tuberculosis |
R93A | 6.5% activity of the wild type enzyme | Mycobacterium tuberculosis |
R93E | 3.6% activity of the wild type enzyme | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WHH9 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WHH9 | - |
- |
Purification (Comment) | Organism |
---|---|
metal-affinity chromatography and Superdex 200 gel filtration chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Mycobacterium tuberculosis | lipoamide + NADH + H+ | - |
? | |
dihydrolipoamide + NAD+ | - |
Mycobacterium tuberculosis H37Rv | lipoamide + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LDH | - |
Mycobacterium tuberculosis |
lipoamide dehydrogenase | - |
Mycobacterium tuberculosis |
LPD | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycobacterium tuberculosis |