Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of both lipdh genes using RT-PCR, expression of the mitochondrial isozyme as GFP-fusion protein giving green fluorescence and of the apicoplast isozyme fused to acyl-carrier-protein resulting in red fluorescence, expression of truncated mitochondrial and apicoplastic isozymes lacking the putative target sequences as His-tagged proteins in Escherichia coli strain BL21-RIL(DE3), the apicoplast isozyme is expressed at very low levels | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ping pong kinetics | Plasmodium falciparum | |
0.021 | - |
NADH | pH 8.0, 25°C, mitochondrial isozyme | Plasmodium falciparum | |
0.045 | - |
NAD+ | pH 8.0, 25°C, mitochondrial isozyme | Plasmodium falciparum | |
0.146 | - |
dihydrolipoamide | pH 8.0, 25°C, mitochondrial isozyme | Plasmodium falciparum | |
0.87 | - |
Lipoamide | pH 8.0, 25°C, mitochondrial isozyme | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | isozyme aLipDH | Plasmodium falciparum | 20011 | - |
mitochondrion | isozyme mLipDH is a component of the branched-chain 2-ketoacid dehydrogenase and the 2-ketoglutarate dehydrogenase multienzyme complexes | Plasmodium falciparum | 5739 | - |
additional information | subcellular localization study using recombinant expression of the isozymes fused to fluorescent protein tags | Plasmodium falciparum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57200 | - |
2 * 57200, mitochondrial enzyme, SDS-PAGE and gel filtration, 2 * 75600, apicoplast enzyme, SDS-PAGE and gel filtration | Plasmodium falciparum |
75600 | - |
2 * 57200, mitochondrial enzyme, SDS-PAGE and gel filtration, 2 * 75600, apicoplast enzyme, SDS-PAGE and gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | Plasmodium falciparum | the forward reaction is the physiological one | lipoamide + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
3D7, 2 lipdh genes encoding 2 isozymes in mitochondrion and apicoplast, both indispensable components of the 2-ketoacid dehydrogenase multienzyme complexes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the apicoplast isozyme contains a potential transit peptide, the mitochondrial isozyme contains targeting sequence | Plasmodium falciparum |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged truncated isozymes from Escherichia coli by nickel affinity chromatography to 98% homogeneity | Plasmodium falciparum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | the mitochondrial isozyme shows ping pong kinetic mechanism | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | both genes are transcribed during erythrocytic development of the parasite | Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Plasmodium falciparum | lipoamide + NADH | - |
r | |
dihydrolipoamide + NAD+ | the forward reaction is the physiological one | Plasmodium falciparum | lipoamide + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57200, mitochondrial enzyme, SDS-PAGE and gel filtration, 2 * 75600, apicoplast enzyme, SDS-PAGE and gel filtration | Plasmodium falciparum |
More | 2 distinct dihydrolipoamide dehydrogenases, both indispensable components of the 2-ketoacid dehydrogenase multienzyme complexes | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Plasmodium falciparum |
LipDH | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
135.5 | 337 | NAD+ | pH 8.0, 25°C, mitochondrial isozyme, forward reaction, varying conditions | Plasmodium falciparum | |
135.5 | 337 | Lipoamide | pH 8.0, 25°C, mitochondrial isozyme, forward reaction, varying conditions | Plasmodium falciparum | |
448 | - |
NADH | pH 8.0, 25°C, mitochondrial isozyme, reverse reaction, varying conditions | Plasmodium falciparum | |
448 | - |
dihydrolipoamide | pH 8.0, 25°C, mitochondrial isozyme, reverse reaction, varying conditions | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reverse reaction, isozyme mLipDH | Plasmodium falciparum |
9 | - |
forward reaction, isozyme LipDH | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | protein-bound | Plasmodium falciparum | |
NAD+ | - |
Plasmodium falciparum | |
NADH | - |
Plasmodium falciparum |