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Literature summary for 1.8.1.4 extracted from

  • Schwinde, J.W.; Hertz, P.F.; Sahm, H.; Eikmanns, B.J.; Guyonvarch, A.
    Lipoamide dehydrogenase from Corynebacterium glutamicum: molecular and physiological analysis of the lpd gene and characterization of the enzyme (2001), Microbiology, 147, 2223-2231.
    View publication on PubMed
Search for more literature for 1.8.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli or in Corynebacterium glutamicum, the cloned gene is expressed in Corynebacterium glutamicum cells harbouring the gene on a plasmid shows 12fold higher specific LPD activity when compared to the wild-type strain Corynebacterium glutamicum

General Stability

General Stability Organism
no loss of activity when the enzyme is frozen at -20°C, and thawed three times Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Corynebacterium glutamicum the enzyme is an essential component of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes ?
-
 ?
additional information Corynebacterium glutamicum DSM 20300 the enzyme is an essential component of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NTE1
-
-
Corynebacterium glutamicum DSM 20300 Q8NTE1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.651
-
-
 Corynebacterium glutamicum

Storage Stability

Storage Stability Organism
4°C or -20°C, stable for at least 1 month Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(p-iodophenyl)-3-p-nitrophenyl-5-phenyltetrazolium chloride + NADH
-
 Corynebacterium glutamicum ? + NAD+
-
 ?
2-(p-iodophenyl)-3-p-nitrophenyl-5-phenyltetrazolium chloride + NADH
-
 Corynebacterium glutamicum DSM 20300 ? + NAD+
-
 ?
dihydrolipoamide + NAD+
-
 Corynebacterium glutamicum lipoamide + NADH
-
 ?
dihydrolipoamide + NAD+
-
 Corynebacterium glutamicum DSM 20300 lipoamide + NADH
-
 ?
additional information the enzyme is an essential component of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes Corynebacterium glutamicum ?
-
 ?
additional information the enzyme is an essential component of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase complexes Corynebacterium glutamicum DSM 20300 ?
-
 ?
oxidized 2,6-dichlorophenolindophenol + NADH
-
 Corynebacterium glutamicum ? + NAD+
-
 ?
oxidized 2,6-dichlorophenolindophenol + NADH
-
 Corynebacterium glutamicum DSM 20300 ? + NAD+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 50 mM phosphate buffer Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Corynebacterium glutamicum
NADH
-
 Corynebacterium glutamicum