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Literature summary for 1.8.1.4 extracted from
- Mycobacterium tuberculosis lipoamide dehydrogenase is encoded by Rv0462 and not by the lpdA or lpdB genes (2001), Biochemistry, 40, 11353-11363.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0041 | - |
NADH | reaction with thio-NAD+ | Mycobacterium tuberculosis |  |
| 0.0073 | - |
NADH | - |
Mycobacterium tuberculosis | |
| 0.0084 | - |
NADH | reaction with O2 | Mycobacterium tuberculosis | |
| 0.036 | - |
thio-NAD+ | reaction with NADH | Mycobacterium tuberculosis | |
| 0.11 | - |
thio-NADH | - |
Mycobacterium tuberculosis | |
| 0.156 | - |
NADH | reaction with 2,6-dimethyl-1,4-benzoquinone | Mycobacterium tuberculosis | |
| 0.161 | - |
NADH | reaction with 5-hydroxy-1,4-naphthoquinone | Mycobacterium tuberculosis | |
| 0.29 | - |
5-hydroxy-1,4-naphthoquinone | - |
Mycobacterium tuberculosis | |
| 0.39 | - |
3-acetylpyridine adenine dinucleotide | - |
Mycobacterium tuberculosis | |
| 0.56 | - |
Lipoamide | reaction with 3-acetylpyridine adenine dinucleotide | Mycobacterium tuberculosis | |
| 0.86 | - |
2,6-dimethyl-1,4-benzoquinone | - |
Mycobacterium tuberculosis | |
| 2.6 | - |
nicotinamide hypoxanthine dinucleotide | - |
Mycobacterium tuberculosis | |
| 5.8 | - |
Lipoamide | reaction with thio-NADH | Mycobacterium tuberculosis | |
| 6.4 | - |
Lipoamide | reaction with nicotinamide hypoxanthine dinucleotide | Mycobacterium tuberculosis | |
| 16 | - |
Lipoamide | reaction with NADH | Mycobacterium tuberculosis | |
| 120 | - |
lipoate | - |
Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Mycobacterium tuberculosis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping-pong mechanism | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-dimethyl-1,4-benzoquinone + NADH | - |
Mycobacterium tuberculosis | 2,6-dimethyl-1,4-benzoquinol + NAD+ | - |
? | |
| 5-hydroxy-1,4-naphthoquinone + NADH | - |
Mycobacterium tuberculosis | 5-hydroxy-1,4-naphthoquinol + NAD+ | - |
? | |
| dihydrolipoamide + NAD+ | - |
Mycobacterium tuberculosis | lipoamide + NADH | - |
? | |
| lipoamide + 3-acetylpyridine adenine dinucleotide | - |
Mycobacterium tuberculosis | dihydrolipoamide + ? | - |
? | |
| lipoamide + nicotinamide hypoxanthine dinucleotide | - |
Mycobacterium tuberculosis | dihydrolipoamide + ? | - |
? | |
| lipoamide + thio-NADH | - |
Mycobacterium tuberculosis | dihydrolipoamide + thio-NAD+ | - |
? | |
| lipoic acid + NADH | - |
Mycobacterium tuberculosis | dihydrolipoic acid + NAD+ | - |
? | |
| additional information | reductive half-reaction, hydride transfer from NADH to FAD, is rate limiting when a quinone is the oxidant | Mycobacterium tuberculosis | ? | - |
? | |
| O2 + NADH | - |
Mycobacterium tuberculosis | ? | - |
? | |
| thio-NAD+ + NADH | - |
Mycobacterium tuberculosis | thio-NADH + NAD+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mycobacterium tuberculosis | |
| NADH | - |
Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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