General Stability | Organism |
---|---|
low ionic strength such as 0.01 M phosphate buffer destroys NADPH-dependent activities | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | - |
Saccharomyces cerevisiae | |
CN- | severe inhibition of NADPH-sulfite,-nitrite,-hydroxylamine reductase, no inhibition of methyl viologen-sulfite reductase, small extent of inhibition of NADPH dependent reduction of cytochrome c, ferricyanide, quinones etc. | Saccharomyces cerevisiae | |
NADP+ | NADPH-dependent activities | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | NADPH-dependent activities | Saccharomyces cerevisiae | |
Sulfide | reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | 0.017 | sulfite | - |
Saccharomyces cerevisiae | |
0.018 | - |
NADPH | reduction of cytochrome c | Saccharomyces cerevisiae | |
0.021 | - |
NADPH | reduction of sulfite | Saccharomyces cerevisiae | |
0.06 | - |
cytochrome c | - |
Saccharomyces cerevisiae | |
0.8 | 1.5 | nitrite | - |
Saccharomyces cerevisiae | |
4.5 | 10.5 | hydroxylamine | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
350000 | - |
calculation from FAD and FMN content, sedimentation coefficient | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + NADPH + H+ | Saccharomyces cerevisiae | - |
sulfide + NADP+ + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | inactivation | Saccharomyces cerevisiae |
Ethanol | inactivation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 0.3 M phosphate buffer pH 7.3, 1 year stable stable | Saccharomyces cerevisiae |
4°C, 0.3 M phosphate buffer pH 7.3, 1 week stable | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADPH | - |
Saccharomyces cerevisiae | ammonia + NADP+ | - |
? | |
additional information | overview on assay methods | Saccharomyces cerevisiae | ? | - |
? | |
additional information | reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 | Saccharomyces cerevisiae | ? | - |
? | |
NADP+ + ferrocytochrome c | - |
Saccharomyces cerevisiae | NADPH + H+ + ferricytochrome c | - |
r | |
nitrite + NADPH | - |
Saccharomyces cerevisiae | ammonia + NADP+ | - |
? | |
sulfite + NADPH | - |
Saccharomyces cerevisiae | sulfide + NADP+ + H2O | - |
? | |
sulfite + NADPH + H+ | - |
Saccharomyces cerevisiae | sulfide + NADP+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | NADPH-sulfite reductase | Saccharomyces cerevisiae |
7.5 | 7.9 | NADPH-cytochrome c reductase | Saccharomyces cerevisiae |
7.7 | 8.5 | methyl viologen-sulfite reductase | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | treatment with ammonium sulfate causes dissociation of FMN but retetion of FAD and heme | Saccharomyces cerevisiae |