Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
Escherichia coli | |
CO | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | 0.017 | sulfite | - |
Escherichia coli | |
0.8 | 1.5 | nitrite | - |
Escherichia coli | |
4.5 | 10.5 | hydroxylamine | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur cluster | hemoprotein subunit contains one siroheme and one Fe4S4 center per polypeptide, heme is in high spin Fe3+ state | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + NADPH | - |
Escherichia coli | ammonia + NADP+ | - |
? | |
additional information | reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit | Escherichia coli | ? | - |
? | |
nitrite + NADPH | NADPH can be replaced by reduced methyl viologen, the latter reaction is catalyzed by hemoprotein subunit alone | Escherichia coli | ammonia + NADP+ | - |
? | |
sulfite + NADPH | NADPH can be replaced by reduced methyl viologen | Escherichia coli | sulfide + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | hemoprotein subunit contains one siroheme and one Fe4S4 center per polypeptide | Escherichia coli |