BRENDA - Enzyme Database
show all sequences of 1.8.1.16

Understanding nicotinamide dinucleotide cofactor and substrate specificity in class I flavoprotein disulfide oxidoreductases: crystallographic analysis of a glutathione amide reductase

van Petegem, F.; de Vos, D.; Savvides, S.; Vergauwen, B.; van Beeumen, J.; J. Mol. Biol. 374, 883-999 (2007)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystal structure of the the enzyme both alone and in complex with NAD+ at 2.1 and 2.5 A resolution, respectively
Marichromatium gracile
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Marichromatium gracile
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
the enzyme forms a dimer, with each monomer consisting of a FAD domain, a Rossmann fold NADH binding domain, and an interface domain
Marichromatium gracile
NAD+
the enzyme forms a dimer, with each monomer consisting of a FAD domain, a Rossmann fold NADH binding domain, and an interface domain
Marichromatium gracile
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
the enzyme forms a dimer, with each monomer consisting of a FAD domain, a Rossmann fold NADH binding domain, and an interface domain
Marichromatium gracile
NAD+
the enzyme forms a dimer, with each monomer consisting of a FAD domain, a Rossmann fold NADH binding domain, and an interface domain
Marichromatium gracile
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystal structure of the the enzyme both alone and in complex with NAD+ at 2.1 and 2.5 A resolution, respectively
Marichromatium gracile
Other publictions for EC 1.8.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
699521
van Petegem
Understanding nicotinamide din ...
Marichromatium gracile
J. Mol. Biol.
374
883-999
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695313
Vergauwen
Crystallization and preliminar ...
Marichromatium gracile
Acta Crystallogr. Sect. D
58
339-340
2002
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698716
Vergauwen
Characterization of glutathion ...
Marichromatium gracile
J. Biol. Chem.
276
20890-20897
2001
-
-
1
-
-
-
-
4
-
-
3
6
-
5
-
-
1
-
-
-
-
-
6
1
-
-
-
4
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
-
-
3
6
-
-
-
1
-
-
-
-
6
1
-
-
-
4
-
-
-
-
-
-
-
-
4
4