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show all sequences of 1.8.1.13

The orphan protein bis-?-glutamylcystine reductase joins the pyridine nucleotide disulfide reductase family

Kim, J.; Copley, S.D.; Biochemistry 52, 2905-2913 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Halobacterium salinarum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
Halobacterium salinarum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halobacterium salinarum
Q9HSN0
-
-
Halobacterium salinarum NRC 1
Q9HSN0
-
-
Purification (Commentary)
Commentary
Organism
both native enzyme and recombinant enzyme, from inclusion bodies
Halobacterium salinarum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
-
724378
Halobacterium salinarum
2 gamma-glutamylcysteine + NADP+
-
-
-
?
bis-gamma-glutamylcystine + NADPH + H+
-
724378
Halobacterium salinarum NRC 1
2 gamma-glutamylcysteine + NADP+
-
-
-
?
additional information
enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity
724378
Halobacterium salinarum
?
-
-
-
-
additional information
enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity
724378
Halobacterium salinarum NRC 1
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
54
-
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
Halobacterium salinarum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Halobacterium salinarum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1
-
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
Halobacterium salinarum
Purification (Commentary) (protein specific)
Commentary
Organism
both native enzyme and recombinant enzyme, from inclusion bodies
Halobacterium salinarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
-
724378
Halobacterium salinarum
2 gamma-glutamylcysteine + NADP+
-
-
-
?
bis-gamma-glutamylcystine + NADPH + H+
-
724378
Halobacterium salinarum NRC 1
2 gamma-glutamylcysteine + NADP+
-
-
-
?
additional information
enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity
724378
Halobacterium salinarum
?
-
-
-
-
additional information
enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity
724378
Halobacterium salinarum NRC 1
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
54
-
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
Halobacterium salinarum
General Information
General Information
Commentary
Organism
physiological function
enzyme is a member of the pyridine nucleotide disulfide oxidoreductase family
Halobacterium salinarum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme is a member of the pyridine nucleotide disulfide oxidoreductase family
Halobacterium salinarum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
49
-
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
Halobacterium salinarum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
49
-
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
Halobacterium salinarum
Other publictions for EC 1.8.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724378
Kim
The orphan protein bis-?-gluta ...
Halobacterium salinarum, Halobacterium salinarum NRC 1
Biochemistry
52
2905-2913
2013
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4
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1
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1
392951
Sundquist
The function of gamma-glutamyl ...
Halobacterium salinarum
J. Biol. Chem.
264
719-725
1989
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1
4
2
1
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2
1
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3
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1
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1
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1
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1
1
1
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1
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1
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1
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4
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2
1
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2
1
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1
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1
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4
1
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1
1
1
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392950
Sundquist
The novel disulfide reductase ...
Halobacterium salinarum
J. Bacteriol.
170
3459-3467
1988
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2
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2
1
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2
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3
1
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2
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3
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3
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2
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2
1
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1
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2
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