BRENDA - Enzyme Database show
show all sequences of 1.8.1.13

The function of gamma-glutamylcysteine and bis-gamma-glutamylcystine reductase in Halobacterium halobium

Sundquist, A.R.; Fahey, R.C.; J. Biol. Chem. 264, 719-725 (1989)

Data extracted from this reference:

General Stability
General Stability
Organism
low-ionic-strength buffer below 1.0 M leads to rapid denaturation
Halobacterium salinarum
Inhibitors
Inhibitors
Commentary
Organism
Structure
AsO2-
25 mM arsenite, relative activity 46%
Halobacterium salinarum
Cu2+
relative activity less than 5%
Halobacterium salinarum
Hg2+
relative activity less than 5%
Halobacterium salinarum
Zn2+
relative activity less than 44%
Halobacterium salinarum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.29
-
NADPH
-
Halobacterium salinarum
0.81
-
bis-gamma-glutamylcystine
-
Halobacterium salinarum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Halobacterium salinarum
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
2 * 61000, SDS-PAGE
Halobacterium salinarum
122000
-
-
Halobacterium salinarum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
Halobacterium salinarum
-
2 gamma-glutamylcysteine + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halobacterium salinarum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Halobacterium salinarum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
28
-
-
Halobacterium salinarum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
392951
Halobacterium salinarum
4-nitrobenzenethiol + NADP+
-
-
-
?
bis-gamma-glutamylcystine + NADPH + H+
-
392951
Halobacterium salinarum
2 gamma-glutamylcysteine + NADP+
-
-
-
?
additional information
no activity detected with GSSG
392951
Halobacterium salinarum
?
-
-
-
-
additional information
lipoic acid disulfide and dihydrolipoamide are no substrates
392951
Halobacterium salinarum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 61000, SDS-PAGE
Halobacterium salinarum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
28.3
-
FAD
-
Halobacterium salinarum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Halobacterium salinarum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.2
about half-maximal activity at pH 6.3 and 8.2
Halobacterium salinarum
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein, 1 mol FAD/mol subunit
Halobacterium salinarum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein, 1 mol FAD/mol subunit
Halobacterium salinarum
General Stability (protein specific)
General Stability
Organism
low-ionic-strength buffer below 1.0 M leads to rapid denaturation
Halobacterium salinarum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AsO2-
25 mM arsenite, relative activity 46%
Halobacterium salinarum
Cu2+
relative activity less than 5%
Halobacterium salinarum
Hg2+
relative activity less than 5%
Halobacterium salinarum
Zn2+
relative activity less than 44%
Halobacterium salinarum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.29
-
NADPH
-
Halobacterium salinarum
0.81
-
bis-gamma-glutamylcystine
-
Halobacterium salinarum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Halobacterium salinarum
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
61000
-
2 * 61000, SDS-PAGE
Halobacterium salinarum
122000
-
-
Halobacterium salinarum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bis-gamma-glutamylcystine + NADPH + H+
Halobacterium salinarum
-
2 gamma-glutamylcysteine + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Halobacterium salinarum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
28
-
-
Halobacterium salinarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
392951
Halobacterium salinarum
4-nitrobenzenethiol + NADP+
-
-
-
?
bis-gamma-glutamylcystine + NADPH + H+
-
392951
Halobacterium salinarum
2 gamma-glutamylcysteine + NADP+
-
-
-
?
additional information
no activity detected with GSSG
392951
Halobacterium salinarum
?
-
-
-
-
additional information
lipoic acid disulfide and dihydrolipoamide are no substrates
392951
Halobacterium salinarum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 61000, SDS-PAGE
Halobacterium salinarum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
28.3
-
FAD
-
Halobacterium salinarum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Halobacterium salinarum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.2
about half-maximal activity at pH 6.3 and 8.2
Halobacterium salinarum
Other publictions for EC 1.8.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724378
Kim
The orphan protein bis-?-gluta ...
Halobacterium salinarum, Halobacterium salinarum NRC 1
Biochemistry
52
2905-2913
2013
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
1
-
-
-
-
-
1
1
-
1
1
392951
Sundquist
The function of gamma-glutamyl ...
Halobacterium salinarum
J. Biol. Chem.
264
719-725
1989
-
-
-
-
-
1
4
2
1
-
2
1
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
1
1
1
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
2
1
-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
392950
Sundquist
The novel disulfide reductase ...
Halobacterium salinarum
J. Bacteriol.
170
3459-3467
1988
-
-
-
-
-
2
-
-
-
-
2
1
-
2
-
-
1
-
-
-
1
1
3
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
1
3
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-