Literature summary for 1.8.1.12 extracted from

Rai, S.; Dwivedi, U.N.; Goyal, N.
Leishmania donovani trypanothione reductase: role of urea and guanidine hydrochloride in modulation of functional and structural properties (2009), Biochim. Biophys. Acta, 1794, 1474-1484.

Search for more literature for 1.8.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Leishmania donovani

General Stability

General Stability	Organism
the loss of activity at lower denaturant (urea) concentrations is not coincided by dimer dissociation or structural unfolding	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
Urea	1 mM urea has no significant effect on activity whereas 50% enzyme inhibition is observed at 750 mM, enzyme after treatment with urea below 500 mM regains about 95% activity	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Leishmania donovani

Renatured (Commentary)

Renatured (Comment)	Organism
8 M urea treated enzyme can also be reactivated up to 50% of native activity	Leishmania donovani

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trypanothione disulfide + NADPH + H+	-	Leishmania donovani	trypanothione + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Leishmania donovani

Synonyms

Synonyms	Comment	Organism
trypanothione reductase	-	Leishmania donovani
TryR	-	Leishmania donovani

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Leishmania donovani
NADPH	-	Leishmania donovani

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Release 2023.1 (January 2023)