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Literature summary for 1.8.1.12 extracted from
- Expression, purification, and characterization of Leishmania donovani trypanothione reductase in Escherichia coli (2005), Protein Expr. Purif., 40, 279-286.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a target for development of antileishmanial drugs | Leishmania donovani |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) as soluble glutathione-S-transferase fusion protein | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Melarsen oxide | over 95% inhibition at 0.05 mM | Leishmania donovani |  |
| additional information | no inhibition by melarsoprol | Leishmania donovani | |
| nifurtimox | - |
Leishmania donovani | |
| nitrofurazone | - |
Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic mechanism | Leishmania donovani | |
| 0.02 | - |
NADPH | pH 7.8, 25°C, recombinant enzyme | Leishmania donovani | |
| 0.05 | - |
trypanothione disulfide | pH 7.8, 25°C, recombinant enzyme | Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54668 | - |
2 * 54668, recombinant enzyme, mass spectrometry and SDS-PAGE | Leishmania donovani |
| 110000 | - |
recombinant enzyme, native PAGE | Leishmania donovani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Leishmania donovani | phylogenetic relationship | ? | - |
? | |
| additional information | Leishmania donovani Dd8 | phylogenetic relationship | ? | - |
? | |
| trypanothione disulfide + NADPH | Leishmania donovani | - |
trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | Leishmania donovani Dd8 | - |
trypanothione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | Q95PT6 | - |
- |
| Leishmania donovani Dd8 | Q95PT6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble glutathione-S-transferase fusion protein from Escherichia coli strain BL21(DE3) to homogeneity, 20fold | Leishmania donovani |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.53 | - |
purified recombinant enzyme | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | phylogenetic relationship | Leishmania donovani | ? | - |
? | |
| additional information | no activity with oxidized glutathione of the recombinant enzyme | Leishmania donovani | ? | - |
? | |
| additional information | phylogenetic relationship | Leishmania donovani Dd8 | ? | - |
? | |
| additional information | no activity with oxidized glutathione of the recombinant enzyme | Leishmania donovani Dd8 | ? | - |
? | |
| trypanothione disulfide + NADPH | - |
Leishmania donovani | trypanothione + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | - |
Leishmania donovani Dd8 | trypanothione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 54668, recombinant enzyme, mass spectrometry and SDS-PAGE | Leishmania donovani |
| More | amino acid sequence of the active site | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TR | - |
Leishmania donovani |
| trypanothione reductase | - |
Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Leishmania donovani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.18 | - |
trypanothione disulfide | pH 7.8, 25°C, recombinant enzyme | Leishmania donovani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | amino acid sequence of the binding site | Leishmania donovani | |
| NADPH | amino acid sequence of the binding site | Leishmania donovani | |
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